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Western blot
Blocking/Neutralizing
Other assayAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Other assay [2]
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- Product number
- PA5-47306 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- FGF6 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- In Western blots, approximately 50% cross-reactivity with recombinant human (rh) FGF-4 is observed, less than 5% cross-reactivity with rhFGF-9 is observed, and less than 2% cross-reactivity with FGF-acidic, rhFGF-5, and rhFGF-7 is observed. In direct ELISAs, less than 1% cross-reactivity with rh beta -ECGF, recombinant mouse (rm) FGF-8b, and rmFGF-8c is observed. Reconstitute at 0.2 mg/mL in sterile PBS. Endoxin level is
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.2 mg/mL
- Storage
- -20°C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Neutralization of FGF6 in NR6R‚3T3 mouse fibroblast cell line. Samples were incubated in FGF6 polyclonal antibody (Product # PA5-47306). Recombinant Human FGF‚6 stimulates proliferation in the NR6R‚3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human FGF‚6 (1 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human FGF‚6 Antigen Affinity-purified Polyclonal Antibody. The ND50 is typically 0.2-1 µg/mL in the presence of heparin (1 µg/mL).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Neutralization of FGF6 in NR6R‚3T3 mouse fibroblast cell line. Samples were incubated in FGF6 polyclonal antibody (Product # PA5-47306). Recombinant Human FGF‚6 stimulates proliferation in the NR6R‚3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human FGF‚6 (1 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human FGF‚6 Antigen Affinity-purified Polyclonal Antibody. The ND50 is typically 0.2-1 µg/mL in the presence of heparin (1 µg/mL).
