AP7608b

antibody from Abcepta

Targeting: EPHA3 ETK, ETK1, HEK, HEK4, TYRO4

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP7608b

Provider

Abcepta

Proper citation

Abgent Cat#AP7608b, RRID:AB_2099226

Product name

EphA3 Antibody (C-term)

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Purified Rabbit Polyclonal Antibody (Pab)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

400 µl

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Abcepta (provider)

Main image

Experimental details

Western blot analysis of anti-EphA3 Pab (Cat. #AP7608b) in CHO cell lysate. EphA3 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

Primary Ab dilution

1:1000

Supportive validation

Submitted by

Abcepta (provider)

Main image

Experimental details

Western blot analysis of EphA3(arrow) using rabbit polyclonal EphA3 Antibody (C-term) (Cat. #AP7608b).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EphA3 gene (Lane 2) (Origene Technologies).

Primary Ab dilution

1:1000

Immunohistochemistry

Supportive validation

Submitted by

Abcepta (provider)

Main image

Experimental details

"Formalin-fixed and paraffin-embedded human placenta tissue reacted with EphA3 antibody (C-term ), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated."

Primary Ab dilution

1:50~100