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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunohistochemistry [1]
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- Product number
- PA5-29904 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- EphA3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: Jurkat, Raji. Predicted reactivity: Mouse (97%), Rat (96%), Pig (97%), Chicken (92%), Bovine (97%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.56 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Eph Receptor A3 using 30 µg of A) Jurkat and B) Raji lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with an Eph Receptor A3 polyclonal antibody (Product # PA5-29904) at a dilution of 1:3000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using EphA3 Polyclonal Antibody (Product # PA5-29904). Whole cell extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with EphA3 Polyclonal Antibody (Product # PA5-29904) diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using EphA3 Polyclonal Antibody (Product # PA5-29904). Mouse tissue extract (50 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EphA3 Polyclonal Antibody (Product # PA5-29904) diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human gastric cancer, using Eph Receptor A3 (Product # PA5-29904) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
