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- Antibody Data
- Antigen structure
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- Product number
- MAB639 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rat Monoclonal EphA2 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse EphA2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human EphA1, recombinant mouse (rm) EphA3, 4, 6, 7, 8, recombinant rat EphA5, rmEphB1, 2, 3, 4, or 6 is observed.
- Reactivity
- Mouse
- Host
- Rat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 500 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Dysregulation of EGFR Pathway in EphA2 Cell Subpopulation Significantly Associates with Poor Prognosis in Colorectal Cancer.
EphA2 receptor mediates increased vascular permeability in lung injury due to viral infection and hypoxia.
Tissue transglutaminase clusters soluble A-type ephrins into functionally active high molecular weight oligomers.
De Robertis M, Loiacono L, Fusilli C, Poeta ML, Mazza T, Sanchez M, Marchionni L, Signori E, Lamorte G, Vescovi AL, Garcia-Foncillas J, Fazio VM
Clinical cancer research : an official journal of the American Association for Cancer Research 2017 Jan 1;23(1):159-170
Clinical cancer research : an official journal of the American Association for Cancer Research 2017 Jan 1;23(1):159-170
EphA2 receptor mediates increased vascular permeability in lung injury due to viral infection and hypoxia.
Cercone MA, Schroeder W, Schomberg S, Carpenter TC
American journal of physiology. Lung cellular and molecular physiology 2009 Nov;297(5):L856-63
American journal of physiology. Lung cellular and molecular physiology 2009 Nov;297(5):L856-63
Tissue transglutaminase clusters soluble A-type ephrins into functionally active high molecular weight oligomers.
Alford SC, Bazowski J, Lorimer H, Elowe S, Howard PL
Experimental cell research 2007 Dec 10;313(20):4170-9
Experimental cell research 2007 Dec 10;313(20):4170-9
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of EphA2 in HEK293 Human Cell Line Transfected with Mouse EphA2 by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with mouse EphA2 was stained with Rat Anti-Mouse EphA2 Monoclonal Antibody (Catalog # MAB639, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B). View our protocol for Staining Membrane-associated Proteins.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of EphA2 in D3 Mouse Cell Line by Flow Cytometry. D3 mouse embryonic stem cell line was stained with Rat Anti-Mouse EphA2 Monoclonal Antibody (Catalog # MAB639, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B). View our protocol for Staining Membrane-associated Proteins.
