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- Antibody Data
- Antigen structure
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- Western blot [1]
- Immunocytochemistry [1]
- Flow cytometry [1]
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- Product number
- DM1226 - Provider product page
- Provider
- OriGene
- Product name
- Eph receptor A2 (EPHA2) mouse monoclonal antibody, clone GM5H5, Purified
- Antibody type
- Monoclonal
- Description
- Eph receptor A2 (EPHA2) mouse monoclonal antibody, clone GM5H5, Purified
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- EPHA2
- Isotype
- IgG
- Antibody clone number
- GM5H5
- Vial size
- 100 µg
- Concentration
- 2.0 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Figure.3: SDS-PAGE analysis of purified EphA2 monoclonal antibody. Lane 1: Molecular weight marker, Lane 2: 2 ug of purified EphA2 antibody. Proteins were separated by SDS-PAGE and stained with RAPID StainTM Reagent.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Figure.2: Spectral Confocal Microscopy of CHO cells using EphA2 antibody . CHO cells were transiently transfected with an expression vector encoding EphA2. Binding of EphA2 was visualized with a FITC-conjugated secondary antibody (green). Actin filaments are labeled with Alexa Fluor-555 Phalloidin (red). Cell nuclei are stained with DAPI (blue).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Figure.1 : FACS analysis of BOSC23 cells using EphA2 antibody . BOSC23 cells were transiently trans-fected with an expression vector encoding either EphA2 (Red curve) or an irrelevant protein (control transfectant). Binding of EphA2 was detected with a PE-conjugated secondary antibody. A positive signal was obtained only with EphA2 transfected cells.
- Validation comment
- FC
