Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PAB3003 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB3003, RRID:AB_1574700
- Product name
- EPHA2 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of EPHA2.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references cDNA cloning and characterization of eck, an epithelial cell receptor protein-tyrosine kinase in the eph/elk family of protein kinases.
Lindberg RA, Hunter T
Molecular and cellular biology 1990 Dec;10(12):6316-24
Molecular and cellular biology 1990 Dec;10(12):6316-24
No comments: Submit comment
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- The EPHA2 polyclonal antibody (Cat # PAB3003) is used in Western blot to detect EPHA2 in CHO cell lysate (Lane 1) and NIH/3T3 cell lysate (Lane 2) .
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with EPHA2 polyclonal antibody (Cat # PAB3003) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Flow cytometric analysis of NCI-H292 cells using EPHA2 polyclonal antibody (Cat # PAB3003)(bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
- Validation comment
- Flow Cytometry