Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Flow cytometry [1]
- Other assay [2]
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- Product number
- PA5-14574 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- EphA2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with non-human primate based on sequence homology.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 1.4 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references NADPH oxidase 4 signaling in a ventilator-induced lung injury mouse model.
microRNA-196b promotes esophageal squamous cell carcinogenesis and chemoradioresistance by inhibiting EPHA7, thereby restoring EPHA2 activity.
Inhibition of EphA2/EphrinA1 signal attenuates lipopolysaccharide-induced lung injury.
Lee SH, Shin MH, Leem AY, Lee SH, Chung KS, Kim YS, Park MS
Respiratory research 2022 Mar 27;23(1):73
Respiratory research 2022 Mar 27;23(1):73
microRNA-196b promotes esophageal squamous cell carcinogenesis and chemoradioresistance by inhibiting EPHA7, thereby restoring EPHA2 activity.
Tan X, Ren S, Fu MZ, Ren S, Yang C, Wu X, Chen T, Latham PS, Meltzer SJ, Fu SW
American journal of cancer research 2021;11(7):3594-3610
American journal of cancer research 2021;11(7):3594-3610
Inhibition of EphA2/EphrinA1 signal attenuates lipopolysaccharide-induced lung injury.
Hong JY, Shin MH, Douglas IS, Chung KS, Kim EY, Jung JY, Kang YA, Kim SK, Chang J, Kim YS, Park MS
Clinical science (London, England : 1979) 2016 Nov 1;130(21):1993-2003
Clinical science (London, England : 1979) 2016 Nov 1;130(21):1993-2003
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis using an EphA2 polyclonal antibody (Product # PA5-14574) in mouse NIH-3T3 cell lysates (35 µg per lane).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of EPHA2 (arrow) using an EphA2 polyclonal antibody (Product # PA5-14574) in 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected with the EPHA2 gene (Lane 2)
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of formalin-fixed, paraffin-embedded human cancer tissue using an EphA2 polyclonal antibody (Product # PA5-14574), followed by HRP-conjugated secondary antibody and AEC staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of NCI-H292 cells using an EphA2 polyclonal antibody (Product # PA5-14574) (bottom), compared to a negative control cell (top) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 2 VILI is decreased in the NOX4 KO mouse and NOX4 inhibitor groups. Western blot of NOX4 ( A ), EphA2, and PI3K 110lambda ( B ). C Densitometry of EphA2, and PI3K 110lambda. *p < 0.05, **p < 0.01, and ***p < 0.001, analyzed by two-way ANOVA with post-hoc testing and Bonferroni correction. KO knockout, HTV high tidal ventilation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 3 NOX4 is associated with EphA2 expression in VILI model. A Total cell counts and protein concentrations. B Identification of cells in BALF with representative visual fields. Cells were mainly macrophages (black arrow) with a few lymphocytes (blue arrow), and neutrophils (red arrow). C Histopathologic features of hematoxylin and eosin-stained mouse lung tissues and lung injury score, showing vascular engorgement, hyaline membrane formation, alveolar wall edema, and inflammatory cell infiltration (200 x magnification). D Western blot of EphA2 and NOX4, and densitometry of EphA2 and NOX4 in response to HTV and EphA2 KO. N = 3 in NVC group and n = 5 in HTV group. *p < 0.05, **p < 0.01, and ***p < 0.001, analyzed by two-way ANOVA with post-hoc testing and Bonferroni correction. BALF bronchoalveolar lavage fluid, WT wild-type, KO knockout, NVC normal ventilation control, HTV high tidal ventilation