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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [1]
- Flow cytometry [1]
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- Product number
- DM1226 - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#DM1226, RRID:AB_10653949
- Product name
- anti EPHA2 / ECK
- Antibody type
- Monoclonal
- Antigen
- Genetic immunisation with cDNA encoding Human EphA2
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- GM5H5
- Vial size
- 0.1 mg
- Concentration
- 2.0 mg/ml
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Figure.2: Spectral Confocal Microscopy of CHO cells using EphA2 antibody (Cat.#DM1226). CHO cells were transiently transfected with an expression vector encoding EphA2. Binding of EphA2 was visualized with a FITC-conjugated secondary antibody (green). Actin filaments are labeled with Alexa Fluor-555 Phalloidin (red). Cell nuclei are stained with DAPI (blue).
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Figure.1: FACS analysis of BOSC23 cells using EphA2 antibody (Cat.#DM1226). BOSC23 cells were transiently trans-fected with an expression vector encoding either EphA2 (Red curve) or an irrelevant protein (control transfectant). Binding of EphA2 was detected with a PE-conjugated secondary antibody. A positive signal was obtained only with EphA2 transfected cells.
