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Western blot
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [1]
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- Product number
- BAF3035 - Provider product page
- Provider
- R&D Systems
- Product name
- Human EphA2 Biotinylated Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human EphA2 in Western blots. In Western blots, approximately 40% cross-reactivity with recombinant mouse (rm) EphA2 is observed and less than 1% cross-reactivity with recombinant human (rh) EphA1, rmEphA4, rhEphA5, rmEphA6, rmEphA7, and rmEphA8 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Biotin
- Antigen sequence
P29317- Isotype
- IgG
- Vial size
- 50 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
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- R&D Systems (provider)
- Main image
- Experimental details
- Detection of EphA2 in A431 Human Cell Line by Flow Cytometry. A431 human epithelial carcinoma cell line was stained with Goat Anti-Human EphA2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF3035, filled histogram) or isotype control antibody (Catalog # BAF108, open histogram), followed by Streptavidin-Phycoerythrin (Catalog # F0040). View our protocol for Staining Membrane-associated Proteins.
