MAB10573

antibody from Abnova Corporation

Targeting: CRK

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB10573

Provider

Abnova Corporation

Proper citation

Product name

CRK monoclonal antibody, clone 3G11E8

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against partial recombinant CRK.

Isotype

IgG

Antibody clone number

3G11E8

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis using CRK monoclonal antibody, clone 3G11E8 (Cat # MAB10573) against HEK293 (1) and CRK-hIgGFc transfected HEK293 (2) cell lysate.

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis using CRK monoclonal antibody, clone 3G11E8 (Cat # MAB10573) against recombinant protein.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

ELISA detection with CRK monoclonal antibody, clone 3G11E8 (Cat # MAB10573).

Validation comment

ELISA

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence analysis of 3T3-L1 cells using CRK monoclonal antibody, clone 3G11E8 (Cat # MAB10573) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human intima cancer (A) and human lung cancer (B) tissues using CRK monoclonal antibody, clone 3G11E8 (Cat # MAB10573) with DAB staining.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Flow cytometry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Flow cytometric analysis of HeLa cells using CRK monoclonal antibody, clone 3G11E8 (Cat # MAB10573) (blue) and negative control (red).

Validation comment

Flow Cytometry