R31526

antibody from NSJ Bioreagents

Targeting: CRK

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

R31526

Provider

NSJ Bioreagents

Product name

Crk Antibody / Proto-oncogene c-Crk

Antibody type

Polyclonal

Description

This highly specific Crk antibody is suitable for use in Western blot/Immunohistochemistry applications with human, mouse and rat samples.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Vial size

100 ug

Concentration

0.5mg/ml if reconstituted with 0.2ml sterile DI water

Storage

After reconstitution, the Crk antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of Crk antibody and Lane 1: rat spleen; 2: (r) thymus; 3: (r) liver; 4: mouse brain; 5: human HeLa; 6: (h) SMMC-7721 lysate. Expected/observed size 34~38KD

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of Crk antibody and recombinant human protein (0.5ng)

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) human 293T, 2) human A549, 3) rat liver and 4) mouse liver tissue lysate with Crk antibody. Expected molecular weight: 34-38 kDa.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC-P: Crk antibody testing of human lung cancer tissue

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC-P: Crk antibody testing of mouse intestine tissue

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC-P: Crk antibody testing of rat brain tissue

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human lung cancer tissue with Crk antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE mouse brain tissue with Crk antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE rat brain tissue with Crk antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.