AM06667SU-N

antibody from Acris Antibodies GmbH

Targeting: CRK

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

AM06667SU-N

Provider

Acris Antibodies GmbH

Proper citation

Product name

anti CRK

Antibody type

Monoclonal

Antigen

Purified recombinant fragment of human CRK expressed in E. Coli.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

3G11C1

Vial size

0.1 ml

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Western blot analysis using CRK mAb against HEK293 (1) and CRK(AA: 1-204)-hIgGFc transfected HEK293 (2) cell lysate.

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Western blot analysis using CRK mAb against human CRK (AA: 1-204) recombinant protein. (Expected MW is 48.4 kDa)

ELISA

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Red: Control Antigen (100ng)Purple: Antigen (10ng)Green: Antigen (50ng)Blue: Antigen (100ng)

Immunocytochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Immunofluorescence analysis of 3T3-L1 cells using CRK mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Immunohistochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using CRK mouse mAb with DAB staining.

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using CRK mouse mAb with DAB staining.

Flow cytometry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Flow cytometric analysis of MCF-7 cells using CRK mouse mAb (blue) and negative control (red).