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Western blot
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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Flow cytometry [1]
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- Product number
- 36-7500 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-Ephrin A3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.25 mg/ml
- Storage
- -20°C
Submitted references A molecular mechanism for the topographic alignment of convergent neural maps.
The Ephrin receptor EphA4 restricts axonal sprouting and enhances branching in the injured mouse optic nerve.
Upregulation of axon guidance molecules in the adult central nervous system of Nogo-A knockout mice restricts neuronal growth and regeneration.
Control of hippocampal dendritic spine morphology through ephrin-A3/EphA4 signaling.
Savier E, Eglen SJ, Bathélémy A, Perraut M, Pfrieger FW, Lemke G, Reber M
eLife 2017 Mar 14;6
eLife 2017 Mar 14;6
The Ephrin receptor EphA4 restricts axonal sprouting and enhances branching in the injured mouse optic nerve.
Joly S, Jordi N, Schwab ME, Pernet V
The European journal of neuroscience 2014 Oct;40(7):3021-31
The European journal of neuroscience 2014 Oct;40(7):3021-31
Upregulation of axon guidance molecules in the adult central nervous system of Nogo-A knockout mice restricts neuronal growth and regeneration.
Kempf A, Montani L, Petrinovic MM, Schroeter A, Weinmann O, Patrignani A, Schwab ME
The European journal of neuroscience 2013 Dec;38(11):3567-79
The European journal of neuroscience 2013 Dec;38(11):3567-79
Control of hippocampal dendritic spine morphology through ephrin-A3/EphA4 signaling.
Murai KK, Nguyen LN, Irie F, Yamaguchi Y, Pasquale EB
Nature neuroscience 2003 Feb;6(2):153-60
Nature neuroscience 2003 Feb;6(2):153-60
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of Ephrin-A3 was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Ephrin-A3 Rabbit Polyclonal Antibody (Product # 36-7500) at 2µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing membrane localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of EPHRIN-A3 was performed using SH-SY5Y cells. Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% Triton™ X-100 for 20 minutes, and blocked with 5% BSA for 30 minutes at room temperature. Cells were labeled with EPHRIN-A3 Rabbit Polyclonal Antibody (Product # 36-7500, red histogram) or with rabbit isotype control (pink histogram) at 3-5 µg/million cells in 2.5% BSA. After incubation at room temperature for 2 hours, the cells were labeled with Alexa Fluor® 488 Goat Anti-Rabbit Secondary Antibody (Product # A11008) at a dilution of 1:400 for 30 minutes at room temperature. The representative 10, 000 cells were acquired and analyzed for each sample using an Attune® Acoustic Focusing Cytometer. The purple histogram represents unstained control cells and the green histogram represents no-primary-antibody control.
