Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- NBP1-18878 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-18878, RRID:AB_1625646
- Product name
- Rabbit Polyclonal Cyclin C Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Cyclin C Antibody [NBP1-18878] - Whole cell lysate (50 ug) from Panc1 (P), 293T (T), Jurkat (J) and H2009 (H) cells for Western Blot. For IP, whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. NBP1-18878 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Cyclin C Antibody [NBP1-18878] - Whole cell lysate (50 ug) from HeLa, HEK293T, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Cyclin C antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: Cyclin C Antibody [NBP1-18878] - Detection of human Cyclin C by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Cyclin C antibody NBP1-18878 used for IP at 6 ug per reaction. For blotting immunoprecipitated Cyclin C, NBP1-18878 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: Cyclin C Antibody [NBP1-18878] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-Cyclin C used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Cyclin C Antibody [NBP1-18878] - Section of human breast carcinoma. Antibody: Affinity purified rabbit anti-Cyclin C used at a dilution of 1:1,000 (1ug/ml). Detection: DAB