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- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunohistochemistry [6]
- Other assay [1]
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- Product number
- PA5-59088 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TGM1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: MMDGPRSDVG RWGGNPLQPP TTPSPEPEPE PDGRSRRGGG RSFWARCCGC CSCRNAADDD WGPEPSDSRG RGSSSGTRRP GSRGSDSRRP VSRGSGVNAA Highest antigen sequence identity to the following orthologs: Mouse - 74%, Rat - 74%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.20 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Trichohyalin-like 1 protein plays a crucial role in proliferation and anti-apoptosis of normal human keratinocytes and squamous cell carcinoma cells.
Makino T, Mizawa M, Yoshihisa Y, Yamamoto S, Tabuchi Y, Miyai M, Hibino T, Sasahara M, Shimizu T
Cell death discovery 2020;6:109
Cell death discovery 2020;6:109
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of TGM1 in human liver using TGM1 Polyclonal Antibody (Product # PA5-59088) shows no positivity in hepatocytes as expected.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of TGM1 in human skin using TGM1 Polyclonal Antibody (Product # PA5-59088) shows moderate membranous positivity in squamous epithelial cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of TGM1 in human colon using TGM1 Polyclonal Antibody (Product # PA5-59088) shows no positivity in glandular cells as expected.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of TGM1 in human esophagus and colon tissues using TGM1 Polyclonal Antibody (Product # PA5-59088). Corresponding TGM1 RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of TGM1 in human esophagus using TGM1 Polyclonal Antibody (Product # PA5-59088) shows moderate to strong membranous positivity in squamous epithelial cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of TGM1 in human colon using TGM1 Polyclonal Antibody (Product # PA5-59088) shows no positivity in glandular cells.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 5 TCHHL1 depletion inhibits proliferation in human epidermal tissue. a Skin-equivalent models were prepared using NHKs transfected with TCHHL1 siRNA or control siRNA. A Hematoxylin-Eosin-stained image is shown. Scale bar: 50 mum. b Skin equivalent models treated TCHHL1 siRNA or control siRNA were stained with anti-Ki67 antibody. The arrowheads indicate Ki67-positive cells. Scale bar: 100 mum. c A quantitative analysis of the Ki67-positive cells in the basal layer. Ki67-positive cells were counted in each 500-mum field (total length 5.0 mm) and expressed as the mean +- SD of three independent experiments. *p < 0.01. d Skin-equivalent models treated with TCHHL1 siRNA or control siRNA were stained with antibodies against filaggrin (FLG), transglutaminase 1 (TGM1), keratin 10 (K10), and keratin 14 (K14). The dotted line indicates the dermo-epidermal junction. Scale bar: 50 mum.
