ABIN2508748

antibody from antibodies-online

Targeting: MAGED1 DLXIN-1, NRAGE

Antibody data

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Validation data

Product number: ABIN2508748 - Provider product page
Provider: antibodies-online
Product name: anti-Melanoma Antigen Family D, 1 (MAGED1) (Center) antibody
Antibody type: Monoclonal
Antigen: This MAGED1 antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between 20-224 amino acids from the Central region of human MAGED1.Antigen type: Recombinant Protein
Description: This antibody is purified through a protein G column, followed by dialysis against PBS.
Reactivity: Human
Host: Mouse
Epitope: Center
Isotype: IgG
Vial size: 200 μL
Storage: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

MAGED1 protein structure - ABIN2508748 shown in red.

Supportive validation

Submitted by: antibodies-online (provider)
Main image
Experimental details: Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain membranes: 1-2. Anti-Nicotinic Acetylcholine Receptor ?2 (extracellular) antibody (ABIN2511196), (1:400). 3-4. Anti-Nicotinic Acetylcholine Receptor ?2 (extracellular) antibody, preincubated with the control peptide antigen. Western blot analysis of human SH-SY5Y neuroblastoma cell lysate: 1. Anti-Nicotinic Acetylcholine Receptor ?2 (extracellular) antibody (ABIN2511196), (1:200). 2. Anti-Nicotinic Acetylcholine Receptor ?2 (extracellular) antibody, preincubated with the control peptide antigen. Expression of nAChR?2 in rat deep cerebellar nucleus Immunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using Anti-Nicotinic Acetylcholine Receptor ?2 (extracellular) antibody (ABIN2511196), (1:100). Staining reveals expression of nAChR?2 (red) in cells with neuronal outline (arrows point at some examples) in the red nucleus. DAPI is used as the counterstain (blue). Expression of Nicotinic Acetylcholine Receptor ?2 in rat PC12 pheochromocytoma cells Immunocytochemical staining of live intact rat PC12 pheochromocytoma cells. A. Extracellular staining of live cells with Anti-Nicotinic Acetylcholine Receptor ?2 (extracellular) antibody (ABIN2511196), (1:50), followed by goat anti-rabbit-AlexaFluor-594 (red). B. Live image of the cells. C. Merge of the two images.

Supportive validation

Submitted by: antibodies-online (provider)
Main image
Experimental details: Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain membranes: 1-2. Anti-Nicotinic Acetylcholine Receptor ?2 (extracellular) antibody (ABIN2511196), (1:400). 3-4. Anti-Nicotinic Acetylcholine Receptor ?2 (extracellular) antibody, preincubated with the control peptide antigen. Western blot analysis of human SH-SY5Y neuroblastoma cell lysate: 1. Anti-Nicotinic Acetylcholine Receptor ?2 (extracellular) antibody (ABIN2511196), (1:200). 2. Anti-Nicotinic Acetylcholine Receptor ?2 (extracellular) antibody, preincubated with the control peptide antigen. Expression of nAChR?2 in rat deep cerebellar nucleus Immunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using Anti-Nicotinic Acetylcholine Receptor ?2 (extracellular) antibody (ABIN2511196), (1:100). Staining reveals expression of nAChR?2 (red) in cells with neuronal outline (arrows point at some examples) in the red nucleus. DAPI is used as the counterstain (blue). Expression of Nicotinic Acetylcholine Receptor ?2 in rat PC12 pheochromocytoma cells Immunocytochemical staining of live intact rat PC12 pheochromocytoma cells. A. Extracellular staining of live cells with Anti-Nicotinic Acetylcholine Receptor ?2 (extracellular) antibody (ABIN2511196), (1:50), followed by goat anti-rabbit-AlexaFluor-594 (red). B. Live image of the cells. C. Merge of the two images.