MA5-34793

antibody from Invitrogen Antibodies

Targeting: CHAF1A CAF-1, CAF1, CAF1B, CAF1P150, MGC71229, P150

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-34793

Provider

Invitrogen Antibodies

Product name

CAF1 p150 Recombinant Rabbit Monoclonal Antibody (JG38-98)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

Positive Control: K562, A431, 293T, LOVO, human tonsil tissue, human colon tissue, Daudi.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Antibody clone number

JG38-98

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles, store in dark

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of p150 CAF1 in K562 (1) and A431 (2) cell lysate. Samples were incubated with p150 CAF1 monoclonal antibody (Product # MA5-34793), at a dilution of 1:1000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of p150 CAF1 in LOVO cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with p150 CAF1 monoclonal antibody (Product # MA5-34793), followed by DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of p150 CAF1 in A431 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with p150 CAF1 monoclonal antibody (Product # MA5-34793), followed by DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of p150 CAF1 in 293T cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with p150 CAF1 monoclonal antibody (Product # MA5-34793), followed by DAPI (blue).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of p150 CAF1 in Daudi cells (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Samples were incubated with p150 CAF1 monoclonal antibody (Product # MA5-34793) at a dilution of 1:100, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG.