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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [5]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- GTX101725 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX101725, RRID:AB_1241032
- Product name
- LTBP4 antibody [N1N2], N-term
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
Submitted references Ltbp4 regulates Pdgfrβ expression via TGFβ-dependent modulation of Nrf2 transcription factor function.
Tomasovic A, Kurrle N, Wempe F, De-Zolt S, Scheibe S, Koli K, Serchinger M, Schnütgen F, Sürün D, Sterner-Kock A, Weissmann N, von Melchner H
Matrix biology : journal of the International Society for Matrix Biology 2017 May;59:109-120
Matrix biology : journal of the International Society for Matrix Biology 2017 May;59:109-120
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- LTBP4 antibody [N1N2], N-term detects LTBP4 protein by western blot analysis.A. 30 ?g K562 whole cell lysate/extract5% SDS-PAGELTBP4 antibody [N1N2], N-term (GTX101725) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (50 ug of whole cell lysate) A: Mouse brain 5% SDS PAGE GTX101725 diluted at 1:1000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Whole cell extract (30 ?g) was separated by 5% SDS-PAGE, and the membrane was blotted with LTBP4 antibody [N1N2], N-term (GTX101725) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- LTBP4 antibody detects LTBP4 protein by Western blot analysis. Mouse tissue extracts (50 £gg) was separated by 5% SDS-PAGE, and the membrane was blotted with LTBP4 antibody (GTX101725) diluted at 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Mouse tissue extract (50 ?g) was separated by 5% SDS-PAGE, and the membrane was blotted with LTBP4 antibody [N1N2], N-term (GTX101725) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of methanol-fixed A431, using LTBP4(GTX101725) antibody at 1:200 dilution.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- LTBP4 antibody [N1N2], N-term detects LTBP4 protein at cytosol on human adrenal gland tumor by immunohistochemical analysis. Sample: Paraffin-embedded adrenal gland tumor. LTBP4 antibody [N1N2], N-term (GTX101725) dilution: 1:250.
