Antibody data
- Antibody Data
- Antigen structure
- References [7]
- Comments [0]
- Validations
- Immunocytochemistry [1]
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- Product number
- ALX-803-321-C100 - Provider product page
- Provider
- Enzo Life Sciences
- Proper citation
- Enzo Life Sciences Cat#ALX-803-321-C100, RRID:AB_2052828
- Product name
- VRK1 (human) (1F6) monoclonal antibody
- Antibody type
- Monoclonal
- Antigen
- Recombinant full length protein
- Description
- Protein G-affinity purified
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 1F6
- Vial size
- 100 μg
- Storage
- -20°C
- Handling
- Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20¡C.
Submitted references Plk3 interacts with and specifically phosphorylates VRK1 in Ser342, a downstream target in a pathway that induces Golgi fragmentation.
A central role for CK1 in catalyzing phosphorylation of the p53 transactivation domain at serine 20 after HHV-6B viral infection.
Proteomics identification of nuclear Ran GTPase as an inhibitor of human VRK1 and VRK2 (vaccinia-related kinase) activities.
Human VRK1 is an early response gene and its loss causes a block in cell cycle progression.
Identification of a dominant epitope in human vaccinia-related kinase 1 (VRK1) and detection of different intracellular subpopulations.
VRK1 signaling pathway in the context of the proliferation phenotype in head and neck squamous cell carcinoma.
p53 Stabilization and accumulation induced by human vaccinia-related kinase 1.
López-Sánchez I, Sanz-García M, Lazo PA
Molecular and cellular biology 2009 Mar;29(5):1189-201
Molecular and cellular biology 2009 Mar;29(5):1189-201
A central role for CK1 in catalyzing phosphorylation of the p53 transactivation domain at serine 20 after HHV-6B viral infection.
MacLaine NJ, Oster B, Bundgaard B, Fraser JA, Buckner C, Lazo PA, Meek DW, Höllsberg P, Hupp TR
The Journal of biological chemistry 2008 Oct 17;283(42):28563-73
The Journal of biological chemistry 2008 Oct 17;283(42):28563-73
Proteomics identification of nuclear Ran GTPase as an inhibitor of human VRK1 and VRK2 (vaccinia-related kinase) activities.
Sanz-García M, López-Sánchez I, Lazo PA
Molecular & cellular proteomics : MCP 2008 Nov;7(11):2199-214
Molecular & cellular proteomics : MCP 2008 Nov;7(11):2199-214
Human VRK1 is an early response gene and its loss causes a block in cell cycle progression.
Valbuena A, López-Sánchez I, Lazo PA
PloS one 2008 Feb 20;3(2):e1642
PloS one 2008 Feb 20;3(2):e1642
Identification of a dominant epitope in human vaccinia-related kinase 1 (VRK1) and detection of different intracellular subpopulations.
Valbuena A, López-Sánchez I, Vega FM, Sevilla A, Sanz-García M, Blanco S, Lazo PA
Archives of biochemistry and biophysics 2007 Sep 1;465(1):219-26
Archives of biochemistry and biophysics 2007 Sep 1;465(1):219-26
VRK1 signaling pathway in the context of the proliferation phenotype in head and neck squamous cell carcinoma.
Santos CR, Rodríguez-Pinilla M, Vega FM, Rodríguez-Peralto JL, Blanco S, Sevilla A, Valbuena A, Hernández T, van Wijnen AJ, Li F, de Alava E, Sánchez-Céspedes M, Lazo PA
Molecular cancer research : MCR 2006 Mar;4(3):177-85
Molecular cancer research : MCR 2006 Mar;4(3):177-85
p53 Stabilization and accumulation induced by human vaccinia-related kinase 1.
Vega FM, Sevilla A, Lazo PA
Molecular and cellular biology 2004 Dec;24(23):10366-80
Molecular and cellular biology 2004 Dec;24(23):10366-80
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Supportive validation
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- ÊWestern BlotÊof endogenous Vaccinia-related Kinase 1 (VRK1) (human) by the MAb to VRK1(1F6) (Prod. No. ALX-803-321). Method: Total cell extracts (10µg) of human embryo kidney 293 (HEK293) (lane 1) and HeLa (lane 2) cell lines were run on a SDS-gel, and revealed with MAb to VRK1(1F6) (1:1'000) for 1 hour at room temperature followed by incubation with HRP-coupled goat-anti mouse antibody (1:4'000). The arrow indicates endogenous VRK1.Ê