GTX84226

antibody from GeneTex

Targeting: LDHA

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX84226

Provider

GeneTex

Proper citation

GeneTex Cat#GTX84226, RRID:AB_10728876

Product name

LDHA antibody [5G10]

Antibody type

Monoclonal

Reactivity

Human

Host

Mouse

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LDHA (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LDHA.

Immunoprecipitation

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunoprecipitation (IP) of LDHA by using TrueMab monoclonal anti-LDHA antibodies (Negative control: IP without adding anti-LDHA antibody.). For each experiment, 500ul of DDK tagged LDHA overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-LDHA antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-LDHA mouse monoclonal antibody. (GTX84226, Dilution 1:50)

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-LDHAmouse monoclonal antibody. (GTX84226, Dilution 1:50)

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Carcinoma of bladder tissue using anti-LDHAmouse monoclonal antibody. (GTX84226, Dilution 1:50)

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Carcinoma of kidney tissue using anti-LDHAmouse monoclonal antibody. (GTX84226, Dilution 1:50)

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Carcinoma of thyroid tissue using anti-LDHAmouse monoclonal antibody. (GTX84226, Dilution 1:50)