LS-C744793

antibody from LSBio

Targeting: LDHA

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Recommended by provider)

Antibody Data

Product number

LS-C744793

Provider

LSBio

Product name

LDHA / LDH1 Antibody (Liquid) LS-C744793

Antibody type

Polyclonal

Description

Delipidation, salt fractionation and ion exchange chromatography followed by dialysis.

Reactivity

Rabbit

Host

Goat

Isotype

IgG

Storage

Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western Blot of Goat Anti-Lactate Dehydrogenase antibody. Lane 1-4: HeLa cell extracts cytoplasmic fraction (CF). Lane 5-8: HeLa cell extracts nuclear fraction (NF). Load: 30 µg per lane. Primary antibody: LDH antibody at 1:400 for overnight at 4°C. Secondary antibody: secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO/TBST overnight at 4°C. Predicted/Observed size: 36.6 kDa, 36 kDa for LDH. Other band(s): None.