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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [2]
- Immunoprecipitation [1]
- Immunohistochemistry [6]
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- Product number
- NBP1-47822 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-47822, RRID:AB_10010801
- Product name
- Mouse Monoclonal Lactate Dehydrogenase A/LDHA Antibody
- Antibody type
- Monoclonal
- Description
- Affinity purified. This antibody is specific for Homo sapiens lactate dehydrogenase A (LDHA), transcript variant 1.
- Reactivity
- Human, Mouse, Rat, Canine, Simian
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references l-Lactate metabolism in HEP G2 cell mitochondria due to the l-lactate dehydrogenase determines the occurrence of the lactate/pyruvate shuttle and the appearance of oxaloacetate, malate and citrate outside mitochondria.
Histone demethylase JMJD2C is a coactivator for hypoxia-inducible factor 1 that is required for breast cancer progression.
Pizzuto R, Paventi G, Porcile C, Sarnataro D, Daniele A, Passarella S
Biochimica et biophysica acta 2012 Sep;1817(9):1679-90
Biochimica et biophysica acta 2012 Sep;1817(9):1679-90
Histone demethylase JMJD2C is a coactivator for hypoxia-inducible factor 1 that is required for breast cancer progression.
Luo W, Chang R, Zhong J, Pandey A, Semenza GL
Proceedings of the National Academy of Sciences of the United States of America 2012 Dec 4;109(49):E3367-76
Proceedings of the National Academy of Sciences of the United States of America 2012 Dec 4;109(49):E3367-76
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Lactate Dehydrogenase A/LDHA Antibody (2D11) [NBP1-47822] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Lactate Dehydrogenase A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Lactate Dehydrogenase A.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Lactate Dehydrogenase A/LDHA Antibody (OTI2D11) [NBP1-47822] - Analysis of extracts (35ug) from 9 different cell lines by using anti-LDHA monoclonal antibody at 1:200 dilution. (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human)
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: Lactate Dehydrogenase A/LDHA Antibody (2D11) [NBP1-47822] - Lane 1: Negative Control Lane 2: NBP1-47824 Lane 3: NBP1-47825 Lane 4: NBP1-47823 Lane 5: NBP1-47822
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Lactate Dehydrogenase A/LDHA Antibody (2D11) [NBP1-47822] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-Lactate Dehydrogenase A mouse monoclonal antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Lactate Dehydrogenase A/LDHA Antibody (2D11) [NBP1-47822] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-Lactate Dehydrogenase A mouse monoclonal antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Lactate Dehydrogenase A/LDHA Antibody (2D11) [NBP1-47822] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-Lactate Dehydrogenase A mouse monoclonal antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Lactate Dehydrogenase A/LDHA Antibody (2D11) [NBP1-47822] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-Lactate Dehydrogenase A mouse monoclonal antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Lactate Dehydrogenase A/LDHA Antibody (2D11) [NBP1-47822] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-Lactate Dehydrogenase A mouse monoclonal antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Lactate Dehydrogenase A/LDHA Antibody (2D11) [NBP1-47822] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-Lactate Dehydrogenase A mouse monoclonal antibody.
