Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Flow cytometry [1]
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Validation data
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- Product number
- NBP1-74023-100ul - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-74023-100ul, RRID:AB_11137336
- Product name
- Mouse Monoclonal Lactate Dehydrogenase A/LDHA Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Lactate Dehydrogenase A/LDHA Antibody (1A4) [NBP1-74023] - Cell lysates of HeLa (35ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human LDHA (1:8000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Lactate Dehydrogenase A/LDHA Antibody (1A4) [NBP1-74023] - Lane 1.: Jurkat cell lysate Lane 2.: 293T cell lysate Lane 3.: A431 cell lysate. antibody (1:1000).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Lactate Dehydrogenase A/LDHA Antibody (1A4) [NBP1-74023] - Flow cytometry analysis of LDHA in HeLa cell line, staining at 2-5ug for 1x106cells. The secondary antibody used goat antimouse IgG Alexa fluor 488 conjugate.