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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [7]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- PA5-23036 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- LDHA Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with bovine and porcine based on 100% sequence homology.
- Reactivity
- Human, Mouse, Bovine, Porcine
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Lactate Dehydrogenase A (LDHA) was performed on NIH/3T3 whole cell lysates using a Lactate Dehydrogenase A polyclonal antibody (Product # PA5-23036).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Lactate Dehydrogenase A (LDHA) was performed on HeLa whole cell lysates using a Lactate Dehydrogenase A polyclonal antibody (Product # PA5-23036).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Lactate Dehydrogenase A (LDHA) was performed by loading 30 µg of normal or diabetic human melanocyte whole cell lysates, treated with 0.4mM palmitate for 0, 4 or 24 hours, per well onto an SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk for 1 hour. The membrane was probed with an LDHA polyclonal antibody (Product # PA5-23036) at a dilution of 1:1000 at 4C overnight, washed in PBST, and probed with an HRP-conjugated anti-rabbit IgG secondary antibody at a dilution of 1:10,000 for 1 hour at room temperature. Detection was performed using a chemiluminescent substrate. Data courtesy of the Innovators Program.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extracts (30 µg lysate) of U-87 MG (Lane 1), PC-3 (Lane 2), A-431 (Lane 3), MCF-7 (Lane 4), DU 145 (Lane 5) MDA-MB-231 (Lane 6) and PANC-1 (Lane 7). The blot was probed with Anti-LDHA Polyclonal Antibody (Product # PA5-23036, 1 µg/mL dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 35 kDa band corresponding to LDHA was observed across the cell lines tested.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of LDHA in NIH/3T3 whole cell lysates. Sample was incubated in LDHA polyclonal antibody (Product # PA5-23036).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of LDHA in HeLa whole cell lysates. Sample was incubated in LDHA polyclonal antibody (Product # PA5-23036).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of LDHA in 0.1 mg/mL HeLa lysate. Samples were incubated in LDHA polyclonal (Product # PA5-23036). This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of LDHA in HeLa cells. Samples were incubated in LDHA polyclonal antibody (Product # PA5-23036).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of LDHA in lung tissue. Samples were incubated in LDHA polyclonal antibody (Product # PA5-23036).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of LDHA. Samples were incubated in LDHA polyclonal antibody (Product # PA5-23036).
