- PA5-26531 - Invitrogen Antibodies LDHA antibody

Zhang X, Li J, Feng Q
Cancer management and research 2021;13:1145-1158

Long DM, Frame AK, Reardon PN, Cumming RC, Hendrix DA, Kretzschmar D, Giebultowicz JM
Aging 2020 Jun 2;12(11):10041-10058

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Western blot analysis using a LDHA polyclonal antibody (Product # PA5-26531) in Ramos, A375 cell lysates (35 µg per lane).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemical analysis of formalin-fixed, paraffin-embedded human skin tissue using a LDHA polyclonal antibody (Product # PA5-26531), followed by HRP-conjugated secondary antibody and DAB staining.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Flow cytometry analysis of HeLa cells using a LDHA polyclonal antibody (Product # PA5-26531) (bottom) compared to a negative control cell (top) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 1 Age-related increase in Ldh expression is associated with elevated LDH protein levels, enzyme activity, and lactate concentration. ( A ) Profile of Ldh mRNA expression in the heads of young (5-days-old) and old (55-days-old) w 1118 flies measured by qRT-PCR. Compared to young, old flies have increased Ldh mRNA levels at each time point with a peak at ZT12. Values are averages of 4 biorepeats reported as a percentage of expression relative to young at ZT0 set to 100%. ( B ) Representative western blot of LDH protein levels in young and old fly heads with tubulin as a loading control. ( C ) Graph showing LDH enzyme activity for both the pyruvate to lactate and lactate to pyruvate reactions in the heads of young (grey bars) and old (blue bars) flies. Enzymatic activity of LDH is higher in the heads of old flies compared to young for the pyruvate to lactate reaction (*p

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Figure 2 Influence of circYY1 inhibition on the malignancy and glycolysis of BC cells. ( A ) Schematic diagram showed 3 siRNAs targeting the back-spliced sequence of circYY1. ( B ) QRT-PCR verified the knockdown efficiency of circYY1 in BT549 and MDA-MB-231 cells. ( C - F ) Influence of circYY1 inhibition on viability, colony formation, migration, and invasion of BT549 and MDA-MB-231 cells was assessed by MTT assay ( C ), colony formation assay ( D ), or transwell assay ( E and F ). ( G - I ) Impacts of circYY1 knockdown on glucose uptake, lactate production, and ATP release in BT549 and MDA-MB-231 cells were determined with corresponding kits. ( J ) Effects of circYY1 silencing on the protein levels of HK2 and LDHA in BT549 and MDA-MB-231 cells were analyzed using Western blotting. ** P < 0.01 and *** P < 0.001.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 4 Effects of miR-769-3p overexpression on the malignancy and glycolysis of BC cells. ( A ) Expression pattern of miR-769-3p in BC tissues and neighboring normal tissues was validated by qRT-PCR. ( B ) Analysis of the correlation between miR-769-3p and circYY1 in BC tissues by Pearson's correlation analysis. ( C ) Analysis of the expression of miR-769-3p in BC cells and MCF10A cells by qRT-PCR. ( D - K ) BT549 and MDA-MB-231 cells were transfected with miR-769-3p mimic and miR-NC. ( D - G ) The viability, colony formation, migration, and invasion of BT549 and MDA-MB-231 cells were analyzed by MTT assay ( D ), colony formation assay ( E ), or transwell assay ( F and G ). ( H - J ) Corresponding kits were used to analyze the levels of glucose uptake, lactate production, and ATP release in BT549 and MDA-MB-231 cells. ( K ) Western blotting presented the protein levels of HK2 and LDHA in BT549 and MDA-MB-231 cells. ** P < 0.01 and *** P < 0.001.

PA5-26531