Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
- Flow cytometry [1]
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Validation data
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- Product number
- TA501007 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA501007, RRID:AB_11140525
- Product name
- Anti-SRR (Serine racemase) mouse monoclonal antibody, clone OTI3B5 (formerly 3B5)
- Antibody type
- Monoclonal
- Description
- Anti-SRR (Serine racemase) mouse monoclonal antibody, clone OTI3B5 (formerly 3B5)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- SRR
- Isotype
- IgG
- Antibody clone number
- OTI3B5
- Vial size
- 100 µl
- Concentration
- 0.5 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SRR (RC210359, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SRR.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SRR monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).(1:200)
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-SRR mouse monoclonal antibody (TA501007) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SRR(RC210359).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded liver tissue within the normal limits using anti-SRR mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501007, Dilution 1:50)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of liver tissue using anti-SRR mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501007, Dilution 1:50)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded pancreas tissue within the normal limits using anti-SRR mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501007, Dilution 1:50)
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either pCMV6-ENTRY SRR(RC210359)(Red) or empty vector control plasmid(Blue) were immunostained with anti-SRR mouse monoclonal(TA501007), and then analyzed by flow cytometry.
- Validation comment
- FC