Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunoprecipitation [1]
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Validation data
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- Product number
- MAB0960 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB0960, RRID:AB_1204615
- Product name
- LIME1 monoclonal antibody, clone LIME-06
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against partial recombinant LIME1.
- Isotype
- IgG
- Antibody clone number
- LIME-06
- Storage
- Store at 4°C. Do not freeze.Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Induction of LIME1 tyrosine phosphorylation. Peripheral blood T cells were left unstimulated (-) or stimulated with anti-human CD3 (MEM-92) or anti-human CD4 (MEM-16), and LIME1 was immunoprecipitated from laurylmaltoside lysates with the LIME1 monoclonal antibody, clone LIME-06 (Cat # MAB0960) (immunoaffinity sorbent) and analyzed by Western blotting to visualize tyrosine-phosphorylated LIME1 (top) and total LIME1 (bottom).
- Validation comment
- Immunoprecipitation