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ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunoprecipitation [1]
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- Product number
- NBP1-44945 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-44945, RRID:AB_10008150
- Product name
- Mouse Monoclonal LIME Antibody
- Antibody type
- Monoclonal
- Description
- Protein A purified. The antibody LIME-06 was raised against intracellular fragment corresponding to aa 141-295 of human LIME, a 30 kDa Lck-interacting transmembrane adaptor expressed by T cells.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1 mg/ml
- Storage
- Store at 4C. Do not freeze.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Immunoprecipitation: LIME Antibody (LIME-06) [NBP1-44945] - Induction of LIME tyrosine phosphorylation. Peripheral blood T cells were left unstimulated (-) or stimulated with anti-human CD3 (MEM-92; cat. no. NB500-521) or anti-human CD4 (MEM-16), and LIME was immunoprecipitated from laurylmaltoside lysates with the LIME-06 antibody (immunoaffinity sorbent) and analyzed by Western blotting to visualize tyrosine-phosphorylated LIME (top) and total LIME (bottom).
