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Immunoprecipitation
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [3]
- Other assay [2]
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- Product number
- MA1-19365 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- LIME Monoclonal Antibody (LIME-06)
- Antibody type
- Monoclonal
- Antigen
- Other
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- LIME-06
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- 4° C, do not freeze
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry intracellular staining pattern of peripheral human whole blood using anti-LIME1 (LIME-06) purified Monoclonal antibody (Product # MA1-19365) (concentration in sample 9 µg/mL, GAM APC).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Separation of human CD3 positive LIME1 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (intracellular staining) of peripheral human whole blood stained using anti-LIME1 (LIME-06) purified Monoclonal antibody (Product # MA1-19365) (concentration in sample 9 µg/mL, GAM APC).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry multicolor intracellular staining of peripheral human whole blood stained using anti-LIME1 (LIME-06) purified Monoclonal antibody (Product # MA1-19365) (concentration in sample 9 µg/mL, GAM APC) and anti-human CD3 (UCHT1) Pacific Blue™ using a dilution of 20 µL reagent/100 µL of peripheral whole blood.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of LIME using a monoclonal antibody (Product # MA1-19365).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Induction of LIME tyrosine phosphorylation. Peripheral blood T cells were left unstimulated (-) or stimulated with anti-human CD3 (MEM-92) or anti-human CD4 (MEM-16), and LIME was immunoprecipitated from laurylmaltoside lysates with the LIME-06 Monoclonal antibody (Product # MA1-19365) (immunoaffinity sorbent) and analyzed by Western blotting to visualize tyrosine-phosphorylated LIME (top) and total LIME (bottom).
