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- Antibody Data
- Antigen structure
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- ELISA [3]
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- Product number
- R20186 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Recombinant Human IgE Antibody
- Antibody type
- Monoclonal
- Description
- This highly specific Recombinant Human IgE antibody is suitable for use in ELISA applications with human samples.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antibody clone number
- RM122
- Vial size
- 100 ug
- Concentration
- 1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
- Storage
- Store the recombinant Human IgE antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Sandwich ELISA with human plasma using recombinant Human IgE antibody as the capture (25ng/well) and biotinylated anti-human light chains (κ+λ) antibody clone RM129 as the detect, followed by an alkaline phosphatase conjugated streptavidin.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- ELISA of hIgs shows recombinant Human IgE antibody reacts only to IgEλ from human myeloma and the IgE from human plasma. No cross reactivity with IgG, IgM, IgD, or IgA.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- ELISA Titration: the plate was coated with different amounts of hIgE. A serial dilution of recombinant Human IgE antibody was used as the primary and an alkaline phosphatase conjugated anti-rabbit IgG as the secondary.
