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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- MA1-24621 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Cardiac Troponin T Monoclonal Antibody (7G7)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- MA1-24621 will not react with mouse, rat, feline, canine, fish, goat, porcine, or rabbit.
- Reactivity
- Human, Mouse, Rat, Bovine
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 7G7
- Vial size
- 200 µg
- Concentration
- 5 mg/mL
- Storage
- 4° C
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot analysis was performed on whole cell extracts (30 µg lysate) of iPSC (Lane 1), iPSC differentiated to Cardiomyocytes (Lane 2), tissue extracts (30 µg lysate) of Mouse Heart (Lane 3), Rat Heart (Lane 4), Mouse Skeletal Muscle (Lane 5) and Rat Skeletal Muscle (Lane 6). The blot was probed with Anti-Cardiac Troponin T Monoclonal Antibody (7G7) (Product # MA1-24621, 1:500 dilution) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/ml, 1:4000 dilution). A 40 kDa band corresponding to Cardiac Troponin T was detected in Cardiomyocytes, Mouse Heart and Rat Heart and not in Mouse and Rat Skeletal Muscle which are reported negative for Cardiac Troponin T expression.
