MA5-17192

antibody from Invitrogen Antibodies

Targeting: TNNT2 CMD1D, CMH2, CMPD2

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-17192

Provider

Invitrogen Antibodies

Product name

Cardiac Troponin T Monoclonal Antibody (1G1)

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Description

MA5-17192 targets troponin T2 in indirect ELISA, FACS and WB applications and shows reactivity with Human samples. The MA5-17192 immunogen is purified recombinant fragment of human troponin T2 expressed in E. Coli. MA5-17192 detects troponin T2 which has a predicted molecular weight of approximately 36kDa.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

1G1

Vial size

100 µL

Concentration

Conc. Not Determined

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Human Cardiac Organoids for Modeling Genetic Cardiomyopathy.

Filippo Buono M, von Boehmer L, Strang J, Hoerstrup SP, Emmert MY, Nugraha B
Cells 2020 Jul 20;9(7)

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of troponin T2 using a troponin T2 monoclonal antibody (Product # MA5-17192) against a human troponin T2 (AA: 88-249) recombinant protein.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of troponin T2 using a troponin T2 monoclonal antibody (Product # MA5-17192) against a human troponin T2 (AA: 88-249) recombinant protein.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometric analysis of MCF-7 cells using troponin T2 monoclonal antibody (Product # MA5-17192) (green) and negative control (red).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 2 Characterization of cardiomyocytes derived from WTSIi020-A and UKKi025-A. ( A , B ) Flow cytometry analysis of cells positive for TNNT2 and OCT4 at the beginning (day 0) and the end (day 15) of differentiation in CDM3. ( C , D ) Median fluorescence intensities of TNNT2 + and OCT4 + populations at day 0 and 15 of the CDM3 protocol. n = 3 biological replicates. ( E , F ) Heatmap plots representing RT-qPCR for markers of pluripotency (NANOG, OCT4, SOX2, KLF4, MYC), cardiac progenitors (NKX2.5) and cardiomyocytes (TNNT2, MHY6, MHY7) on day 0 and day 15. n = 3 independent biological replicates performed in technical triplicates. ( G ) Immunofluorescent staining images of cardiomyocytes derived from WTSIi020-A and UKKi025-A. WTSIi020-A CMs show clear formation of immature sarcomeric-like structure while UKKi025-A CMs display a disrupted structure and rather enlarged cell morphology (marked with *). Significance level A : **** p oct4 < 0.001; * p tnnt2 < 0.023; B : *** p oct4 = 0.0002; **** p tnnt2 < 0.0001; C : ** p oct4 = 0.0015; **** p tnnt2 < 0.0001; D : * p oct4 = 0.0142; **** p tnnt2 < 0.0001; E : p