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- Antibody Data
- Antigen structure
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- Product number
- NB200-412 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB200-412, RRID:AB_2272095
- Product name
- Mouse Monoclonal Troponin T Type 2 (cardiac) Antibody
- Antibody type
- Monoclonal
- Description
- Protein A purified. This is specific for cadiac troponin. Does not cross-react with skeletal muscle troponin T, cTnI and TnC.
- Reactivity
- Human, Mouse, Rat, Canine
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.2 mg
- Storage
- Store at 4C. Do not freeze.
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- Experimental details
- Sandwich ELISA: Troponin T Type 2 (cardiac) Antibody (1C11) [NB200-412] - Calibrating curves for sandwich cTnT fluoroimmunoassay with different animal TnTs as antigen: human, canine, rat, mouse Clone 1C11 for capture (1 ug/well) and Clone b 7E7 for detection (200 ng/well). Assay time: 30 min. Room temperature. Hybridoma cell line producing Clone 7E7 was raised after hybridization of myeloma cell line Sp2/0 with splenocytes of mice immunized with human tertiary Tn complex. Thus Clone 7E7 demonstrates slight cross-reaction with cTnI (about 5% based on ELISA data).
