MA1-16687

antibody from Invitrogen Antibodies

Targeting: TNNT2 CMD1D, CMH2, CMPD2

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

MA1-16687

Provider

Invitrogen Antibodies

Product name

Cardiac Troponin T Monoclonal Antibody (1C11)

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Description

This antibody is useful for Troponin T immunoassay.

Antibody clone number

1C11

Concentration

5.7 mg/mL

References

Submitted references

Identification of a dihydropyridine scaffold that blocks ryanodine receptors.

Gunaratne GS, Rebbeck RT, McGurran LM, Yan Y, Arzua T, Frolkis T, Sprague DJ, Bai X, Cornea RL, Walseth TF, Marchant JS
iScience 2022 Jan 21;25(1):103706

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on whole cell extracts (30 µg lysate) of iPSC (Lane 1), iPSC differentiated to Cardiomyocytes (Lane 2), tissue extracts (30 µg lysate) of Mouse Heart (Lane 3), Rat Heart (Lane 4), Mouse Skeletal Muscle (Lane 5), Rat Skeletal Muscle (Lane 6) and Mouse Brain (Lane 7). The blot was probed with Anti-Cardiac Troponin T Monoclonal Antibody (1C11) (Product # MA1-16687, 1:500 dilution) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/ml, 1:4000 dilution). A 40 kDa band corresponding to Cardiac Troponin T was detected in Cardiomyocytes, Mouse Heart and Rat Heart and not in Mouse and Rat Skeletal Muscle and Mouse Brain which are reported negative for Cardiac Troponin T expression.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on whole cell extracts (30 µg lysate) of iPSC (Lane 1), iPSC differentiated to Cardiomyocytes (Lane 2), tissue extracts (30 µg lysate) of Mouse Heart (Lane 3), Rat Heart (Lane 4), Mouse Skeletal Muscle (Lane 5), Rat Skeletal Muscle (Lane 6) and Mouse Brain (Lane 7). The blot was probed with Anti-Cardiac Troponin T Monoclonal Antibody (1C11) (Product # MA1-16687, 1:500 dilution) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/ml, 1:4000 dilution). A 40 kDa band corresponding to Cardiac Troponin T was detected in Cardiomyocytes, Mouse Heart and Rat Heart and not in Mouse and Rat Skeletal Muscle and Mouse Brain which are reported negative for Cardiac Troponin T expression.

ELISA

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

ELISA of Cardiac Troponin T in animal TnTs as antigen: human, canine, rat, mouse Clone 1C11 for capture (1 µg/well) and Clone b 7E7 for detection (200 ng/well). Samples were incubated in Cardiac Troponin T monoclonal antibody (Product # MA1-16687). Assay time: 30 min. Room temperature. Hybridoma cell line producing Clone 7E7 was raised after hybridization of myeloma cell line Sp2/0 with splenocytes of mice immunized with human tertiary Tn complex. Thus Clone 7E7 demonstrates slight cross-reaction with cTnI (about 5% based on ELISA data).