sc-55549

ELANE antibody from Santa Cruz Biotechnology

ELA2, HLE, HNE, NE

Western blot

Supportive data in Antibodypedia

Immunohistochemistry

Supportive data in Antibodypedia

Provider product page for sc-55549

Antibody Data

Product number

sc-55549

Provider

Santa Cruz Biotechnology

Proper citation

Santa Cruz Biotechnology Cat#sc-55549, RRID:AB_831596

Product name

Anti-ELA2

Provider product page

Antibody type

Monoclonal

Reactivity

Human

Host

Mouse

Western blot

Supportive validation

Submitted by

per

Main image

Experimental details

Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.

Validation comment

No bands detected.

Primary Ab dilution

1:500

Secondary Ab dilution

1:7000

Lane 1

Marker [kDa]: 250, 130, 95, 72, 55, 36, 28, 17, 11

Lane 2

RT-4

Lane 3

U-251MG sp

Lane 4

Human Plasma

Lane 5

Liver

Lane 6

Tonsil

Theoretical target weight

[kDa] 29

Immunohistochemistry

Supportive validation

Submitted by

per

Main image

Experimental details

Immunohistochemical staining of human bone marrow shows strong cytoplasmic positivity in a subset of bone marrow poietic cells.

Validation comment

Two independent antibodies targeting one protein yielding similar staining patterns. Staining pattern consistent with experimental and/or bioinformatic data.