HPA000496

CCNB3 antibody from Atlas Antibodies

Eligible for validation within the Antibodypedia Validation Initiative

Immunocytochemistry

Supportive data in Antibodypedia

Immunohistochemistry

Enhanced validation

Provider product page for HPA000496

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Antibody Data

Product number

HPA000496

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA000496, RRID:AB_611422

Product name

Anti-CCNB3

Provider product page

Antibody type

Polyclonal

Description

Affinity purified using the PrEST antigen as affinity ligand

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

KMCASQRKQSCQEESLAVQDVNMEEDSFFMESMSF
KKKPKTEESIPTHKLSSLKKKCTIYGKICHFRKPP
VLQTTICGAMSSIKKPTTEKETLFQELSVLQEKHT
TEHEMS


Isotype

IgG

Vial size

100 µl

Storage

Store at +4¡C for short term storage. Long time storage is recommended at -20¡C.

References

BCOR-CCNB3 fusions are frequent in undifferentiated sarcomas of male children.
Peters TL, Kumar V, Polikepahad S, Lin FY, Sarabia SF, Liang Y, Wang WL, Lazar AJ, Doddapaneni H, Chao H, Muzny DM, Wheeler DA, Okcu MF, Plon SE, Hicks MJ, López-Terrada D, Parsons DW, Roy A
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc 2015 Apr;28(4):575-86

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A new subtype of bone sarcoma defined by BCOR-CCNB3 gene fusion.
Pierron G, Tirode F, Lucchesi C, Reynaud S, Ballet S, Cohen-Gogo S, Perrin V, Coindre JM, Delattre O
Nature genetics 2012 Mar 4;44(4):461-6

Immunocytochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line A-431 shows localization to nuclear speckles.

Sample type

HUMAN

Immunohistochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human testis shows strong nuclear positivity in a subset of cells in seminiferous ducts.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human liver shows no positivity in hepatocytes as expected.

Sample type

HUMAN

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Immunohistochemistry analysis in human testis and liver tissues using HPA000496 antibody. Corresponding CCNB3 RNA-seq data are presented for the same tissues.

Sample type

HUMAN

Orthogonal method

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

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