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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- Ab092954 - Provider product page
- Provider
- Aladdin Scientific
- Product name
- Casein Kinase 1 delta/CSNK1D Mouse mAb
- Antibody type
- Monoclonal
- Description
- Mouse anti Human Casein Kinase 1 delta/CSNK1D Antibody, Monoclonal(C3), could be used for WB, IHC and so on . Application: WB: 0.01-3 µg/mL IHC: 5-30 µg/mL Protein Function Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Central component of the circadian clock. May act as a negative regulator of circadian rhythmicity by phosphorylating PER1 and PER2. Retains PER1 in the cytoplasm.
- Reactivity
- Human, Rat, Porcine
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
AA 1-409- Antibody clone number
- C3
- Vial size
- 100_l,10_l,1ml,20_l,50_l
- Concentration
- Lot by Lot
- Storage
- Store at 4_ short term (1-2 weeks). Store at -20_ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
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Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Casein Kinase 1 delta/CSNK1D Mouse mAb (Ab092954) - Western Blot All lanes: Casein Kinase 1 delta/CSNK1D Mouse mAb (Ab092954) at 1/2000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution Predicted band size: 47 kDa Observed band size: 45 kDa Exposure time: 246.0 s
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Casein Kinase 1 delta/CSNK1D Mouse mAb (Ab092954) - IHC DAB staining on IHC Sample: Human Cerebrum Tissue Primary Ab: 30 µg/mL Casein Kinase 1 delta/CSNK1D Mouse mAb (Ab092954) Control: Used PBS instead of primary antibody Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
