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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunohistochemistry [1]
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- Product number
- PA5-47852 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ASCL2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Reconstitute in sterile PBS to a final concentration of 0.2 mg/mL.
- Reactivity
- Human
- Host
- Sheep
- Isotype
- IgG
- Vial size
- 100 μg
- Concentration
- 0.2 mg/mL
- Storage
- -20°C, Avoid Freeze/Thaw Cycles
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- Invitrogen Antibodies (provider)
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- Experimental details
- Immunohistochemical analysis of ASCL2 in immersion fixed paraffin-embedded sections of human intestine. Samples were incubated in ASCL2 polyclonal antibody (Product # PA5-47852) using a dilution of 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hemotoxylin (blue). Lower panel shows a lack of labeling when primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. Specific staining was localized to the base of intestinal crypts.
