44-530G

antibody from Invitrogen Antibodies

Targeting: EIF2B5 EIF-2B, EIF2Bepsilon

Western blot (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

44-530G

Provider

Invitrogen Antibodies

Product name

Phospho-eIF2b epsilon (Ser539) Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Storage

-20°C

References

Submitted references

Proximal tubular epithelial insulin receptor mediates high-fat diet-induced kidney injury.

Lee HJ, Mariappan MM, Norton L, Bakewell T, Feliers D, Oh SB, Donati A, Rubannelsonkumar CS, Venkatachalam MA, Harris SE, Rubera I, Tauc M, Ghosh Choudhury G, Kahn CR, Sharma K, DeFronzo RA, Kasinath BS
JCI insight 2021 Feb 8;6(3)

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Pharmacological inhibition of GSK-3 in a guinea pig model of LPS-induced pulmonary inflammation: II. Effects on skeletal muscle atrophy.

Verhees KJ, Pansters NA, Baarsma HA, Remels AH, Haegens A, de Theije CC, Schols AM, Gosens R, Langen RC
Respiratory research 2013 Nov 1;14(1):117

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Glycogen synthase kinase-3β is required for the induction of skeletal muscle atrophy.

Verhees KJ, Schols AM, Kelders MC, Op den Kamp CM, van der Velden JL, Langen RC
American journal of physiology. Cell physiology 2011 Nov;301(5):C995-C1007

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Nutrient provision increases signalling and protein synthesis in human skeletal muscle after repeated sprints.

Coffey VG, Moore DR, Burd NA, Rerecich T, Stellingwerff T, Garnham AP, Phillips SM, Hawley JA
European journal of applied physiology 2011 Jul;111(7):1473-83

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Pulmonary artery smooth muscle hypertrophy: roles of glycogen synthase kinase-3beta and p70 ribosomal S6 kinase.

Deng H, Hershenson MB, Lei J, Anyanwu AC, Pinsky DJ, Bentley JK
American journal of physiology. Lung cellular and molecular physiology 2010 Jun;298(6):L793-803

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Ectopic expression of eIF2Bepsilon in rat skeletal muscle rescues the sepsis-induced reduction in guanine nucleotide exchange activity and protein synthesis.

Tuckow AP, Vary TC, Kimball SR, Jefferson LS
American journal of physiology. Endocrinology and metabolism 2010 Aug;299(2):E241-8

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Resistance exercise increases muscle protein synthesis and translation of eukaryotic initiation factor 2Bepsilon mRNA in a mammalian target of rapamycin-dependent manner.

Kubica N, Bolster DR, Farrell PA, Kimball SR, Jefferson LS
The Journal of biological chemistry 2005 Mar 4;280(9):7570-80

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 2 Muscle protein turnover signaling is not affected following chronic LPS-treatment and GSK-3 inhibition. Protein synthesis and protein degradation-related signaling molecules were determined in whole muscle homogenates of the extensor digitorum longus (EDL) muscles by Western blot analysis of guinea pigs that were treated intranasally with LPS or SB216763 for 12 weeks. Representative immunoblots depict protein levels of (A) phospho-Akt (p-Akt), total Akt, phospho-GSK-3beta (p-GSK-3beta), total GSK-3beta, phospho-eukaryotic initiation factor 2B (p-eIF2B), GAPDH, (B) phospho-mammalian target of rapamycin (p-mTOR), total mTOR, phospho-p70S6K (p-p70S6K), total p70S6K, phospho-S6 (p-S6), phospho-4E-BP1 (p-4E-BP1), total 4E-BP1, GAPDH, (C) phospho-FoXO1 (p-FoXO1), total FoXO1, phospho-FoXO3a (p-FoXO3a), total FoXO3a and GAPDH. The accompanying bar graphs show the densitometric analysis results (means +- SEM, n = 6), represented as a percentage of the vc control group corrected for GAPDH. All data is shown as a ratio of phospho- to total protein for each target (except p-eIF2B, p-S6 and (p-) 4E-BP1). *p < 0.05 compared with the vc control group. NS: not significant.