A302-557A

antibody from Invitrogen Antibodies

Targeting: EIF2B5 EIF-2B, EIF2Bepsilon

Western blot (Recommended by provider)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

A302-557A

Provider

Invitrogen Antibodies

Product name

eIF2B5 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

4° C

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human eIF2B5 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-eIF2B5 (Cat. No. A302-557A) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human eIF2B5 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-eIF2B5 antibody A302-557A used for IP at 10 µg/mg lysate. eIF2B5 was also immunoprecipitated by rabbit anti-eIF2B5 antibody A302-556A, which recognizes an upstream epitope. For blotting immunoprecipitated eIF2B5, A302-556A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.