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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Other assay [1]
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- Product number
- PA5-19601 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-eIF2b epsilon Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- PA5-19601 targets eIF2B epsilon in IF and WB applications and shows reactivity with Human, mouse, and Rat samples. The PA5-19601 immunogen is synthetic peptide conjugated to KLH derived from within residues 550 - 650 of Human eIF2B epsilon. PA5-19601 detects eIF2B epsilon which has a predicted molecular weight of approximately 75 kDa.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 200 µL
- Concentration
- 0.1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MEF1 Whole Cell Lysate using Product # PA5-19601, eIF2B epsilon primary antibody at a dilution of 1:250 (lane 1). Staining of Mouse Brain Tissue Lysate at a dilution of 1:250 (lane 2). Blot treated with a secondary IR Dye680-conjugated Goat polyclonal anti-Rabbit antibody was used at a dilution of 1:10000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of HeLa using Product # PA5-19601, anti-eIF2B epsilon antibody. The cells were fixed with PFA (4%) for 10 minutes, permabilised with BSA (1%), normal goat serum (10%) and glycine (0.3 M) in 0.1% T-BST for 20 minutes and exposed to the primary antibody at a concentration of 1 µg/mL for 1 hour at room temp. The secondary antibody was a 448 fluorescence conjugated Goat anti-rabbit IgG (green) at a dilution of 1:1000. A WGA- 594 fluorescent conjugated stain was used to label plasma membranes (red) and the nuclei stain was DAPI (blue).
