GTX30808

antibody from GeneTex

Targeting: EIF2B5 EIF-2B, EIF2Bepsilon

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX30808

Provider

GeneTex

Proper citation

GeneTex Cat#GTX30808, RRID:AB_1952718

Product name

EIF2B epsilon antibody

Antibody type

Polyclonal

Reactivity

Human, Mouse, Rat

Host

Rabbit

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Lysates from various cell lines were separated on SDS-PAGE and probed with 1:500 dilution of Rabbit Anti-eIF2B£` (Cat. No. GTX30808). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjμgate and a chemiluminiscence substrate. Lanes: 1. 293-T; 2. HeLa; 3. MCF-7; 4. A549; 5. A431; 6. 3T3; 7. C2; 8. RAT1; 9. PC12

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Anti-eIF2Be (GTX30808): Immunofluorescence of HUVEC cells using Anti-eIF2Be (red) at a 1:50 dilution, taken at 40¡Ñ magnification and nuclear staining with Hoescht 33342 (blue). Yale HTCB IF procedure used.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Localization of eIF2B£` in NIH-3T3 cytoplasm. Cells were stained with Rabbit Anti-eIF2B£` (GTX30808) at a dilution of 1:1,000 followed by Goat Anti-Rabbit IgG, Cy3 conjμgate, and counterstained with DAPI (blue) to stain nuclei (x40 magnification).

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Anti-eIF2Be (GTX30808): Intensity analysis of Anti-eIF2Be staining. Cytoplasmic and nuclear intensity values were obtained at 8 different antibody dilutions (1:50 to 1:6,400) as compared with the negative control.