Antibody data
- Antibody Data
- Antigen structure
- References [10]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- 13791-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#13791-1-AP, RRID:AB_2195683
- Product name
- Spartin, SPG20 antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated Spartin, SPG20 antibody (Cat. #13791-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: FC, IHC, IP, WB,ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references A novel mutation in SPART gene causes a severe neurodevelopmental delay due to mitochondrial dysfunction with complex I impairments and altered pyruvate metabolism.
Novel Homozygous Missense Mutation in SPG20 Gene Results in Troyer Syndrome Associated with Mitochondrial Cytochrome c Oxidase Deficiency.
Germline deletion of huntingtin causes male infertility and arrested spermiogenesis in mice.
Different expression levels of spartin cause broad spectrum of cellular consequences in human neuroblastoma cells.
Chromosome mis-segregation and cytokinesis failure in trisomic human cells.
The role of spartin and its novel ubiquitin binding region in DALIS occurrence.
Spg20-/- mice reveal multimodal functions for Troyer syndrome protein spartin in lipid droplet maintenance, cytokinesis and BMP signaling.
Spartin recruits PKC-ζ via the PKC-ζ-interacting proteins ZIP1 and ZIP3 to lipid droplets.
SPG20, a novel biomarker for early detection of colorectal cancer, encodes a regulator of cytokinesis.
Developmental and degenerative features in a complicated spastic paraplegia.
Diquigiovanni C, Bergamini C, Diaz R, Liparulo I, Bianco F, Masin L, Baldassarro VA, Rizzardi N, Tranchina A, Buscherini F, Wischmeijer A, Pippucci T, Scarano E, Cordelli DM, Fato R, Seri M, Paracchini S, Bonora E
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2019 Oct;33(10):11284-11302
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2019 Oct;33(10):11284-11302
Novel Homozygous Missense Mutation in SPG20 Gene Results in Troyer Syndrome Associated with Mitochondrial Cytochrome c Oxidase Deficiency.
Spiegel R, Soiferman D, Shaag A, Shalev S, Elpeleg O, Saada A
JIMD reports 2017;33:55-60
JIMD reports 2017;33:55-60
Germline deletion of huntingtin causes male infertility and arrested spermiogenesis in mice.
Yan J, Zhang H, Liu Y, Zhao F, Zhu S, Xie C, Tang TS, Guo C
Journal of cell science 2016 Feb 1;129(3):492-501
Journal of cell science 2016 Feb 1;129(3):492-501
Different expression levels of spartin cause broad spectrum of cellular consequences in human neuroblastoma cells.
Milewska M, Byrne PC
Cell biology international 2015 Sep;39(9):1007-15
Cell biology international 2015 Sep;39(9):1007-15
Chromosome mis-segregation and cytokinesis failure in trisomic human cells.
Nicholson JM, Macedo JC, Mattingly AJ, Wangsa D, Camps J, Lima V, Gomes AM, Dória S, Ried T, Logarinho E, Cimini D
eLife 2015 May 5;4
eLife 2015 May 5;4
The role of spartin and its novel ubiquitin binding region in DALIS occurrence.
Karlsson AB, Washington J, Dimitrova V, Hooper C, Shekhtman A, Bakowska JC
Molecular biology of the cell 2014 Apr;25(8):1355-65
Molecular biology of the cell 2014 Apr;25(8):1355-65
Spg20-/- mice reveal multimodal functions for Troyer syndrome protein spartin in lipid droplet maintenance, cytokinesis and BMP signaling.
Renvoisé B, Stadler J, Singh R, Bakowska JC, Blackstone C
Human molecular genetics 2012 Aug 15;21(16):3604-18
Human molecular genetics 2012 Aug 15;21(16):3604-18
Spartin recruits PKC-ζ via the PKC-ζ-interacting proteins ZIP1 and ZIP3 to lipid droplets.
Urbanczyk A, Enz R
Journal of neurochemistry 2011 Sep;118(5):737-48
Journal of neurochemistry 2011 Sep;118(5):737-48
SPG20, a novel biomarker for early detection of colorectal cancer, encodes a regulator of cytokinesis.
Lind GE, Raiborg C, Danielsen SA, Rognum TO, Thiis-Evensen E, Hoff G, Nesbakken A, Stenmark H, Lothe RA
Oncogene 2011 Sep 15;30(37):3967-78
Oncogene 2011 Sep 15;30(37):3967-78
Developmental and degenerative features in a complicated spastic paraplegia.
Manzini MC, Rajab A, Maynard TM, Mochida GH, Tan WH, Nasir R, Hill RS, Gleason D, Al Saffar M, Partlow JN, Barry BJ, Vernon M, LaMantia AS, Walsh CA
Annals of neurology 2010 Apr;67(4):516-25
Annals of neurology 2010 Apr;67(4):516-25
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 13791-1-AP(Spartin, SPG20 antibody) at dilution of 1:1000
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells, using SPG20 antibody 13791-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- IP Result of anti-Spartin, SPG20 (IP:13791-1-AP, 3ug; Detection:13791-1-AP 1:1000) with mouse brain tissue lysate 6000ug.
- Sample type
- tissue
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human gliomas using 13791-1-AP(Spartin, SPG20 antibody) at dilution of 1:100 (under 10x lens)
- Sample type
- tissue
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human gliomas using 13791-1-AP(Spartin, SPG20 antibody) at dilution of 1:100 (under 40x lens)
- Sample type
- tissue