11010-1-AP

antibody from Proteintech Group

Targeting: GABARAPL1 APG8L, ATG8B, ATG8L, gec1

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

11010-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#11010-1-AP, RRID:AB_2294415

Product name

GABARAPL1-Specific antibody

Antibody type

Polyclonal

Description

KD/KO validated GABARAPL1-Specific antibody (Cat. #11010-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: FC, IF, IHC, IP, WB, ELISA.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

A TBK1 variant causes autophagolysosomal and motoneuron pathology without neuroinflammation in mice.

Brenner D, Sieverding K, Srinidhi J, Zellner S, Secker C, Yilmaz R, Dyckow J, Amr S, Ponomarenko A, Tunaboylu E, Douahem Y, Schlag JS, Rodríguez Martínez L, Kislinger G, Niemann C, Nalbach K, Ruf WP, Uhl J, Hollenbeck J, Schirmer L, Catanese A, Lobsiger CS, Danzer KM, Yilmazer-Hanke D, Münch C, Koch P, Freischmidt A, Fetting M, Behrends C, Parlato R, Weishaupt JH
The Journal of experimental medicine 2024 May 6;221(5)

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Machine learning-based identification and immune characterization of ferroptosis-related molecular clusters in osteoarthritis and validation.

Guo X, Feng X, Yang Y, An W, Bai L
Aging 2024 May 29;16(11):9437-9459

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D-pinitol alleviates diabetic cardiomyopathy by inhibiting the optineurin-mediated endoplasmic reticulum stress and glycophagy signaling pathway.

Li X, Yu X, Yu F, Fu C, Zhao W, Liu X, Dai C, Gao H, Cheng M, Li B
Phytotherapy research : PTR 2024 Mar;38(3):1681-1694

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GABARAPL1 is essential in extracellular vesicle cargo loading and metastasis development.

Beaumont JEJ, Ju J, Barbeau LMO, Demers I, Savelkouls KG, Derks K, Bouwman FG, Wauben MHM, Zonneveld MI, Keulers TGH, Rouschop KMA
Radiotherapy and oncology : journal of the European Society for Therapeutic Radiology and Oncology 2024 Jan;190:109968

---

Necrosulfonamide causes oxidation of PCM1 and impairs ciliogenesis and autophagy.

Renaud CCN, Nicolau CA, Maghe C, Trillet K, Jardine J, Escot S, David N, Gavard J, Bidère N
iScience 2024 Apr 19;27(4):109580

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Assessment of Autophagy Markers Suggests Increased Activity Following LVAD Therapy.

Martin TG, Juarros MA, Cleveland JC Jr, Bristow MR, Ambardekar AV, Buttrick PM, Leinwand LA
JACC. Basic to translational science 2023 Sep;8(9):1043-1056

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Analysis of ATG4C function in vivo.

Tamargo-Gómez I, Martínez-García GG, Suárez MF, Mayoral P, Bretones G, Astudillo A, Prieto-Lloret J, Sveen C, Fueyo A, Engedal N, López-Otín C, Mariño G
Autophagy 2023 Nov;19(11):2912-2933

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An atypical GABARAP binding module drives the pro-autophagic potential of the AML-associated NPM1c variant.

Mende H, Khatri A, Lange C, Poveda-Cuevas SA, Tascher G, Covarrubias-Pinto A, Löhr F, Koschade SE, Dikic I, Münch C, Bremm A, Brunetti L, Brandts CH, Uckelmann H, Dötsch V, Rogov VV, Bhaskara RM, Müller S
Cell reports 2023 Dec 26;42(12):113484

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The centrosomal protein 131 participates in the regulation of mitochondrial apoptosis.

Renaud CCN, Trillet K, Jardine J, Merlet L, Renoult O, Laurent-Blond M, Catinaud Z, Pecqueur C, Gavard J, Bidère N
Communications biology 2023 Dec 15;6(1):1271

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The ATG8 Family Proteins GABARAP and GABARAPL1 Target Antigen to Dendritic Cells to Prime CD4(+) and CD8(+) T Cells.

Fonderflick L, Baudu T, Adotévi O, Guittaut M, Adami P, Delage-Mourroux R
Cells 2022 Sep 6;11(18)

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Acrolein inhalation acutely affects the regulation of mitochondrial metabolism in rat lung.

Tulen CBM, Snow SJ, Leermakers PA, Kodavanti UP, van Schooten FJ, Opperhuizen A, Remels AHV
Toxicology 2022 Mar 15;469:153129

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Liver glycogen phosphorylase is upregulated in glioblastoma and provides a metabolic vulnerability to high dose radiation.

Zois CE, Hendriks AM, Haider S, Pires E, Bridges E, Kalamida D, Voukantsis D, Lagerholm BC, Fehrmann RSN, den Dunnen WFA, Tarasov AI, Baba O, Morris J, Buffa FM, McCullagh JSO, Jalving M, Harris AL
Cell death & disease 2022 Jun 28;13(6):573

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Hypoxia-induced mitochondrial abnormalities in cells of the placenta.

Vangrieken P, Al-Nasiry S, Bast A, Leermakers PA, Tulen CBM, Janssen GMJ, Kaminski I, Geomini I, Lemmens T, Schiffers PMH, van Schooten FJ, Remels AHV
PloS one 2021;16(1):e0245155

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Comprehensive Analysis of the Tumor Microenvironment and Ferroptosis-Related Genes Predict Prognosis with Ovarian Cancer.

Li XX, Xiong L, Wen Y, Zhang ZJ
Frontiers in genetics 2021;12:774400

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ATG4D is the main ATG8 delipidating enzyme in mammalian cells and protects against cerebellar neurodegeneration.

Tamargo-Gómez I, Martínez-García GG, Suárez MF, Rey V, Fueyo A, Codina-Martínez H, Bretones G, Caravia XM, Morel E, Dupont N, Cabo R, Tomás-Zapico C, Souquere S, Pierron G, Codogno P, López-Otín C, Fernández ÁF, Mariño G
Cell death and differentiation 2021 Sep;28(9):2651-2672

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GABARAPL1 Inhibits EMT Signaling through SMAD-Tageted Negative Feedback.

Jacquet M, Hervouet E, Baudu T, Herfs M, Parratte C, Feugeas JP, Perez V, Reynders C, Ancion M, Vigneron M, Baguet A, Guittaut M, Fraichard A, Despouy G
Biology 2021 Sep 24;10(10)

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Subtractive CRISPR screen identifies the ATG16L1/vacuolar ATPase axis as required for non-canonical LC3 lipidation.

Ulferts R, Marcassa E, Timimi L, Lee LC, Daley A, Montaner B, Turner SD, Florey O, Baillie JK, Beale R
Cell reports 2021 Oct 26;37(4):109899

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Atg5 Regulates Selective Autophagy of the Parental Macronucleus during Tetrahymena Sexual Reproduction.

Bo T, Kang Y, Liu Y, Xu J, Wang W
Cells 2021 Nov 8;10(11)

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In vivo 5-ethynyluridine (EU) labelling detects reduced transcription in Purkinje cell degeneration mouse mutants, but can itself induce neurodegeneration.

Van't Sant LJ, White JJ, Hoeijmakers JHJ, Vermeij WP, Jaarsma D
Acta neuropathologica communications 2021 May 21;9(1):94

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Hemizygous deletion of Tbk1 worsens neuromuscular junction pathology in TDP-43(G298S) transgenic mice.

Sieverding K, Ulmer J, Bruno C, Satoh T, Tsao W, Freischmidt A, Akira S, Wong PC, Ludolph AC, Danzer KM, Lobsiger CS, Brenner D, Weishaupt JH
Experimental neurology 2021 Jan;335:113496

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GABARAP suppresses EMT and breast cancer progression via the AKT/mTOR signaling pathway.

Liu Y, Wang D, Lei M, Gao J, Cui Y, Jin X, Yu Q, Jiang Y, Guo Y, Liu Y, Cai L, Chen X
Aging 2021 Feb 11;13(4):5858-5874

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Effect of dihydromyricetin on hepatic encephalopathy associated with acute hepatic failure in mice.

Cheng L, Wang X, Ma X, Xu H, Yang Y, Zhang D
Pharmaceutical biology 2021 Dec;59(1):557-564

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Secretion of pro-angiogenic extracellular vesicles during hypoxia is dependent on the autophagy-related protein GABARAPL1.

Keulers TG, Libregts SF, Beaumont JEJ, Savelkouls KG, Bussink J, Duimel H, Dubois L, Zonneveld MI, López-Iglesias C, Bezstarosti K, Demmers JA, Vooijs M, Wauben M, Rouschop KMA
Journal of extracellular vesicles 2021 Dec;10(14):e12166

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Haploinsufficiency of TANK-binding kinase 1 prepones age-associated neuroinflammatory changes without causing motor neuron degeneration in aged mice.

Bruno C, Sieverding K, Freischmidt A, Satoh T, Walther P, Mayer B, Ludolph AC, Akira S, Yilmazer-Hanke D, Danzer KM, Lobsiger CS, Brenner D, Weishaupt JH
Brain communications 2020;2(2):fcaa133

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The Electrophysiological Determinants of Corticospinal Motor Neuron Vulnerability in ALS.

Jara JH, Sheets PL, Nigro MJ, Perić M, Brooks C, Heller DB, Martina M, Andjus PR, Ozdinler PH
Frontiers in molecular neuroscience 2020;13:73

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CASP9 (caspase 9) is essential for autophagosome maturation through regulation of mitochondrial homeostasis.

An HK, Chung KM, Park H, Hong J, Gim JE, Choi H, Lee YW, Choi J, Mun JY, Yu SW
Autophagy 2020 Sep;16(9):1598-1617

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Cytoprotective autophagy induction by withaferin A in prostate cancer cells involves GABARAPL1.

Hahm ER, Singh SV
Molecular carcinogenesis 2020 Oct;59(10):1105-1115

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A conserved ATG2-GABARAP family interaction is critical for phagophore formation.

Bozic M, van den Bekerom L, Milne BA, Goodman N, Roberston L, Prescott AR, Macartney TJ, Dawe N, McEwan DG
EMBO reports 2020 Mar 4;21(3):e48412

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Pulmonary inflammation-induced alterations in key regulators of mitophagy and mitochondrial biogenesis in murine skeletal muscle.

Leermakers PA, Remels AHV, Langen RCJ, Schols AMWJ, Gosker HR
BMC pulmonary medicine 2020 Jan 21;20(1):20

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Triggering a switch from basal- to luminal-like breast cancer subtype by the small-molecule diptoindonesin G via induction of GABARAPL1.

Fan M, Chen J, Gao J, Xue W, Wang Y, Li W, Zhou L, Li X, Jiang C, Sun Y, Wu X, Wu X, Ge H, Shen Y, Xu Q
Cell death & disease 2020 Aug 15;11(8):635

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A DNM2 Centronuclear Myopathy Mutation Reveals a Link between Recycling Endosome Scission and Autophagy.

Puri C, Manni MM, Vicinanza M, Hilcenko C, Zhu Y, Runwal G, Stamatakou E, Menzies FM, Mamchaoui K, Bitoun M, Rubinsztein DC
Developmental cell 2020 Apr 20;53(2):154-168.e6

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GABARAPL1 Promotes AR+ Prostate Cancer Growth by Increasing FL-AR/AR-V Transcription Activity and Nuclear Translocation.

Su B, Zhang L, Liu S, Chen X, Zhang W
Frontiers in oncology 2019;9:1254

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Redundancy of human ATG4 protease isoforms in autophagy and LC3/GABARAP processing revealed in cells.

Agrotis A, Pengo N, Burden JJ, Ketteler R
Autophagy 2019 Jun;15(6):976-997

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Heterozygous Tbk1 loss has opposing effects in early and late stages of ALS in mice.

Brenner D, Sieverding K, Bruno C, Lüningschrör P, Buck E, Mungwa S, Fischer L, Brockmann SJ, Ulmer J, Bliederhäuser C, Philibert CE, Satoh T, Akira S, Boillée S, Mayer B, Sendtner M, Ludolph AC, Danzer KM, Lobsiger CS, Freischmidt A, Weishaupt JH
The Journal of experimental medicine 2019 Feb 4;216(2):267-278

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Evaluating expression of autophagy-related genes in oriental river prawn Macrobrachium nipponense as potential biomarkers for hypoxia exposure.

Sun S, Wu Y, Fu H, Yang M, Ge X, Zhu J, Xuan F, Wu X
Ecotoxicology and environmental safety 2019 Apr 30;171:484-492

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Rapid induction of p62 and GABARAPL1 upon proteasome inhibition promotes survival before autophagy activation.

Sha Z, Schnell HM, Ruoff K, Goldberg A
The Journal of cell biology 2018 May 7;217(5):1757-1776

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Distinct roles for motor neuron autophagy early and late in the SOD1(G93A) mouse model of ALS.

Rudnick ND, Griffey CJ, Guarnieri P, Gerbino V, Wang X, Piersaint JA, Tapia JC, Rich MM, Maniatis T
Proceedings of the National Academy of Sciences of the United States of America 2017 Sep 26;114(39):E8294-E8303

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Full length RTN3 regulates turnover of tubular endoplasmic reticulum via selective autophagy.

Grumati P, Morozzi G, Hölper S, Mari M, Harwardt MI, Yan R, Müller S, Reggiori F, Heilemann M, Dikic I
eLife 2017 Jun 15;6

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GABARAPL1 tumor suppressive function is independent of its conjugation to autophagosomes in MCF-7 breast cancer cells.

Poillet-Perez L, Jacquet M, Hervouet E, Gauthier T, Fraichard A, Borg C, Pallandre JR, Gonzalez BJ, Ramdani Y, Boyer-Guittaut M, Delage-Mourroux R, Despouy G
Oncotarget 2017 Aug 22;8(34):55998-56020

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Proximity Ligation In situ Assay is a Powerful Tool to Monitor Specific ATG Protein Interactions following Autophagy Induction.

Gauthier T, Claude-Taupin A, Delage-Mourroux R, Boyer-Guittaut M, Hervouet E
PloS one 2015;10(6):e0128701

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GABARAPL1 is required for increased EGFR membrane expression during hypoxia.

Keulers TG, Schaaf MB, Peeters HJ, Savelkouls KG, Vooijs MA, Bussink J, Jutten B, Rouschop KM
Radiotherapy and oncology : journal of the European Society for Therapeutic Radiology and Oncology 2015 Sep;116(3):417-22

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Gabarapl1 mediates androgen-regulated autophagy in prostate cancer.

Xie CW, Zhou Y, Liu SL, Fang ZY, Su B, Zhang W
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 2015 Nov;36(11):8727-33

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Low expression of GABARAPL1 is associated with a poor outcome for patients with hepatocellular carcinoma.

Liu C, Xia Y, Jiang W, Liu Y, Yu L
Oncology reports 2014 May;31(5):2043-8

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The role of GABARAPL1/GEC1 in autophagic flux and mitochondrial quality control in MDA-MB-436 breast cancer cells.

Boyer-Guittaut M, Poillet L, Liang Q, Bôle-Richard E, Ouyang X, Benavides GA, Chakrama FZ, Fraichard A, Darley-Usmar VM, Despouy G, Jouvenot M, Delage-Mourroux R, Zhang J
Autophagy 2014 Jun;10(6):986-1003

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Protein degradation and quality control in cells from laforin and malin knockout mice.

Garyali P, Segvich DM, DePaoli-Roach AA, Roach PJ
The Journal of biological chemistry 2014 Jul 25;289(30):20606-14

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Cardiomyocyte glycophagy is regulated by insulin and exposure to high extracellular glucose.

Mellor KM, Varma U, Stapleton DI, Delbridge LM
American journal of physiology. Heart and circulatory physiology 2014 Apr 15;306(8):H1240-5

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Coordinate regulation of mature dopaminergic axon morphology by macroautophagy and the PTEN signaling pathway.

Inoue K, Rispoli J, Yang L, Macleod D, Beal MF, Klann E, Abeliovich A
PLoS genetics 2013;9(10):e1003845

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Specific distribution of the autophagic protein GABARAPL1/GEC1 in the developing and adult mouse brain and identification of neuronal populations expressing GABARAPL1/GEC1.

Le Grand JN, Bon K, Fraichard A, Zhang J, Jouvenot M, Risold PY, Boyer-Guittaut M, Delage-Mourroux R
PloS one 2013;8(5):e63133

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Myocardial glycophagy - a specific glycogen handling response to metabolic stress is accentuated in the female heart.

Reichelt ME, Mellor KM, Curl CL, Stapleton D, Delbridge LM
Journal of molecular and cellular cardiology 2013 Dec;65:67-75

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ATG8 family proteins act as scaffolds for assembly of the ULK complex: sequence requirements for LC3-interacting region (LIR) motifs.

Alemu EA, Lamark T, Torgersen KM, Birgisdottir AB, Larsen KB, Jain A, Olsvik H, Øvervatn A, Kirkin V, Johansen T
The Journal of biological chemistry 2012 Nov 16;287(47):39275-90

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Identification of HSP90 as a new GABARAPL1 (GEC1)-interacting protein.

Seguin-Py S, Lucchi G, Croizier S, Chakrama FZ, Despouy G, Le Grand JN, Ducoroy P, Boireau W, Boyer-Guittaut M, Jouvenot M, Fraichard A, Delage-Mourroux R
Biochimie 2012 Mar;94(3):748-58

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AMPK promotes skeletal muscle autophagy through activation of forkhead FoxO3a and interaction with Ulk1.

Sanchez AM, Csibi A, Raibon A, Cornille K, Gay S, Bernardi H, Candau R
Journal of cellular biochemistry 2012 Feb;113(2):695-710

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The p97/VCP ATPase is critical in muscle atrophy and the accelerated degradation of muscle proteins.

Piccirillo R, Goldberg AL
The EMBO journal 2012 Aug 1;31(15):3334-50

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Temporal orchestration of circadian autophagy rhythm by C/EBPβ.

Ma D, Panda S, Lin JD
The EMBO journal 2011 Sep 6;30(22):4642-51

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Starch-binding domain-containing protein 1 (Stbd1) and glycogen metabolism: Identification of the Atg8 family interacting motif (AIM) in Stbd1 required for interaction with GABARAPL1.

Jiang S, Wells CD, Roach PJ
Biochemical and biophysical research communications 2011 Sep 30;413(3):420-5

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GABARAPL1 antibodies: target one protein, get one free!

Le Grand JN, Chakrama FZ, Seguin-Py S, Fraichard A, Delage-Mourroux R, Jouvenot M, Risold PY, Boyer-Guittaut M
Autophagy 2011 Nov;7(11):1302-7

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Starch binding domain-containing protein 1/genethonin 1 is a novel participant in glycogen metabolism.

Jiang S, Heller B, Tagliabracci VS, Zhai L, Irimia JM, DePaoli-Roach AA, Wells CD, Skurat AV, Roach PJ
The Journal of biological chemistry 2010 Nov 5;285(45):34960-71

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Autophagy is essential for mouse sense of balance.

Mariño G, Fernández AF, Cabrera S, Lundberg YW, Cabanillas R, Rodríguez F, Salvador-Montoliu N, Vega JA, Germanà A, Fueyo A, Freije JM, López-Otín C
The Journal of clinical investigation 2010 Jul;120(7):2331-44

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Markers of autophagy are downregulated in failing human heart after mechanical unloading.

Kassiotis C, Ballal K, Wellnitz K, Vela D, Gong M, Salazar R, Frazier OH, Taegtmeyer H
Circulation 2009 Sep 15;120(11 Suppl):S191-7

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FoxO transcription factors promote autophagy in cardiomyocytes.

Sengupta A, Molkentin JD, Yutzey KE
The Journal of biological chemistry 2009 Oct 9;284(41):28319-28331

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FoxO3 coordinately activates protein degradation by the autophagic/lysosomal and proteasomal pathways in atrophying muscle cells.

Zhao J, Brault JJ, Schild A, Cao P, Sandri M, Schiaffino S, Lecker SH, Goldberg AL
Cell metabolism 2007 Dec;6(6):472-83

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

rat brain tissue were subjected to SDS PAGE followed by western blot with 11010-1-AP(ATG8L antibody) at dilution of 1:1000

Sample type

tissue

Immunocytochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

The GABARAPL1 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human GABARAPL1. This antibody recognizes human,mouse,rat antigen. The GABARAPL1 antibody has been validated for the following applications: ELISA, WB, IHC, IP, IF analysis.

Appendix

11010-1-AP.pdf

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Immunoprecipitation

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

IP Result of anti-ATG8L (IP:11010-1-AP, 3ug; Detection:11010-1-AP 1:500) with mouse liver tissue lysate 5000ug.

Sample type

tissue

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human ovary tumor using 11010-1-AP(ATG8L antibody) at dilution of 1:50 (under 10x lens)

Sample type

tissue