Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- Ab104445 - Provider product page
- Provider
- Aladdin Scientific
- Product name
- GABARAPL1 Antibody
- Antibody type
- Polyclonal
- Description
- Rabbit anti Human GABARAPL1 Antibody, Polyclonal, could be used for WB, IHC, Flow and so on.Application:WB: 1/1000IHC: 1/100-1/500Flow: 1/10-1/50Protein FunctionUbiquitin-like modifier that increases cell-surface expression of kappa-type opioid receptor through facilitating anterograde intracellular trafficking of the receptor. Involved in formation of autophagosomal vacuoles. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation.Post-translationalThe precursor molecule is cleaved by ATG4B to form the cytosolic form, GABARAPL1-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, GABARAPL1-II (By similarity). ATG4B also mediates the delipidation required for GABARAPL1 recycling when autophagosomes fuse with lysosomes. The Legionella effector RavZ is a deconjugating enzyme that produces an ATG8 product that would be resistant to reconjugation by the host machinery due to the cleavage of the reactive C-terminal glycine.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antigen sequence
AA 1-117
- Vial size
- 100μl,10μl,1ml,50μl
- Concentration
- 0,5 mg/ml
- Storage
- Store at 4°C short term (1-2 weeks). Store at -20°C long term (24months). Upon delivery aliquot. Avoid freeze/thaw cycle.
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Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- GABARAPL1 Antibody (Ab104445) - Western Blot All lanes: GABARAPL1 Antibody (Ab104445) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 14 kDa Observed band size: 16, 18 kDa Exposure time: 100.0 s
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- GABARAPL1 Antibody (Ab104445) - IHC Formalin-fixed and paraffin-embedded human brain tissue reacted with GABARAPL1 Antibody (Ab104445), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- GABARAPL1 Antibody (Ab104445) - Flow GABARAPL1 Antibody (Ab104445) flow cytometric analysis of HeLa cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis