GTX104590

antibody from GeneTex

Targeting: RCC1 CHC1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX104590

Provider

GeneTex

Proper citation

GeneTex Cat#GTX104590, RRID:AB_1951671

Product name

RCC1 antibody

Antibody type

Polyclonal

Reactivity

Human, Mouse

Host

Rabbit

References

Submitted references

Methylation-silencing RCC1 expression is associated with tumorigenesis and depth of invasion in gastric cancer.

Lin YL, Chen HL, Cheng SB, Yeh DC, Huang CC, P'eng FK, Tsai TC, Wu CC, Chen CM
International journal of clinical and experimental pathology 2015;8(11):14257-69

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

RCC1 antibody detects RCC1 protein by Western blot analysis.A. 30 ?g Neuro2A whole cell lysate/extract B. 30 ?g GL261 whole cell lysate/extract12 % SDS-PAGERCC1 antibody (GTX104590) dilution: 1:1000

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample(30£gg whole cell lysate)A: 293TB: A431 (GTX27909)C: H1299D: HeLa S3 (GTX14654)E: Hep G2 (GTX27900)F: MOLT4 (GTX27912)G: Raji (GTX27908) 10% SDS PAGEGTX104590 diluted at 1:1000

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

RCC1 antibody detects RCC1 protein at nucleus by immunofluorescent analysis.Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: RCC1 protein stained by RCC1 antibody (GTX104590) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded KYSE170 xenograft, using RCC1(GTX104590) antibody at 1:500 dilution.