Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- GTX104590 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX104590, RRID:AB_1951671
- Product name
- RCC1 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
Submitted references Methylation-silencing RCC1 expression is associated with tumorigenesis and depth of invasion in gastric cancer.
Lin YL, Chen HL, Cheng SB, Yeh DC, Huang CC, P'eng FK, Tsai TC, Wu CC, Chen CM
International journal of clinical and experimental pathology 2015;8(11):14257-69
International journal of clinical and experimental pathology 2015;8(11):14257-69
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- RCC1 antibody detects RCC1 protein by Western blot analysis.A. 30 ?g Neuro2A whole cell lysate/extract B. 30 ?g GL261 whole cell lysate/extract12 % SDS-PAGERCC1 antibody (GTX104590) dilution: 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample(30£gg whole cell lysate)A: 293TB: A431 (GTX27909)C: H1299D: HeLa S3 (GTX14654)E: Hep G2 (GTX27900)F: MOLT4 (GTX27912)G: Raji (GTX27908) 10% SDS PAGEGTX104590 diluted at 1:1000
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- RCC1 antibody detects RCC1 protein at nucleus by immunofluorescent analysis.Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: RCC1 protein stained by RCC1 antibody (GTX104590) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded KYSE170 xenograft, using RCC1(GTX104590) antibody at 1:500 dilution.