sc-55589

PIK3CD antibody from Santa Cruz Biotechnology

p110D

Western blot

Supportive data in Antibodypedia

Immunohistochemistry

Supportive data in Antibodypedia

Provider product page for sc-55589

Antibody Data

Product number

sc-55589

Provider

Santa Cruz Biotechnology

Proper citation

Santa Cruz Biotechnology Cat#sc-55589, RRID:AB_831654

Product name

Anti-PIK3CD

Provider product page

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Reactivity

Human

Host

Mouse

References

Modification of integration site preferences of an HIV-1-based vector by expression of a novel synthetic protein.
Silvers RM, Smith JA, Schowalter M, Litwin S, Liang Z, Geary K, Daniel R
Human gene therapy 2010 Mar;21(3):337-49

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Evidence that the Nijmegen breakage syndrome protein, an early sensor of double-strand DNA breaks (DSB), is involved in HIV-1 post-integration repair by recruiting the ataxia telangiectasia-mutated kinase in a process similar to, but distinct from, cellular DSB repair.
Smith JA, Wang FX, Zhang H, Wu KJ, Williams KJ, Daniel R
Virology journal 2008 Jan 22;5:11

Western blot

Supportive validation

Submitted by

per

Main image

Experimental details

Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.

Validation comment

Single band corresponding to the predicted size in kDa (+/-20%).

Primary Ab dilution

1:500

Secondary Ab dilution

1:7000

Lane 1

Marker [kDa]: 229, 107, 78, 46, 31, 26, 16.2

Lane 2

RT-4

Lane 3

U-251MG sp

Lane 4

Human Plasma

Lane 5

Liver

Lane 6

Tonsil

Theoretical target weight

[kDa] 122

Immunohistochemistry

Supportive validation

Submitted by

per

Main image

Experimental details

Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in neuronal cells.

Validation comment

Staining pattern partly consistent with experimental and/or bioinformatic data.