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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [4]
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- Validations
- Western blot [1]
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- Product number
- HPA021799 - Provider product page
- Provider
- Atlas Antibodies
- Proper citation
- Atlas Antibodies Cat#HPA021799, RRID:AB_1849841
- Product name
- Anti-GOLGA2
- Antibody type
- Polyclonal
- Description
- Polyclonal Antibody against Human GOLGA2, Gene description: golgin A2, Alternative Gene Names: GM130, golgin-95, Validated applications: ICC, IHC, WB, Uniprot ID: Q08379, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 0.2 mg/ml
- Storage
- Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Handling
- The antibody solution should be gently mixed before use.
Submitted references Histone methyltransferase SUV39H1 regulates the Golgi complex via the nuclear envelope-spanning LINC complex
The SUN2-nesprin-2 LINC complex and KIF20A function in the Golgi dispersal
Systematic mapping of genetic interactions for de novo fatty acid synthesis identifies C12orf49 as a regulator of lipid metabolism
Wdpcp, a PCP Protein Required for Ciliogenesis, Regulates Directional Cell Migration and Cell Polarity by Direct Modulation of the Actin Cytoskeleton
Roy A, Nishino M, Imaizumi H, Yokoyama Y, Katahira J, Kimura H, Matsuura N, Matsumura M
PLOS ONE 2023;18(7):e0283490
PLOS ONE 2023;18(7):e0283490
The SUN2-nesprin-2 LINC complex and KIF20A function in the Golgi dispersal
Hieda M, Matsumoto T, Isobe M, Kurono S, Yuka K, Kametaka S, Wang J, Chi Y, Kameda K, Kimura H, Matsuura N, Matsuura S
Scientific Reports 2021;11(1)
Scientific Reports 2021;11(1)
Systematic mapping of genetic interactions for de novo fatty acid synthesis identifies C12orf49 as a regulator of lipid metabolism
Aregger M, Lawson K, Billmann M, Costanzo M, Tong A, Chan K, Rahman M, Brown K, Ross C, Usaj M, Nedyalkova L, Sizova O, Habsid A, Pawling J, Lin Z, Abdouni H, Wong C, Weiss A, Mero P, Dennis J, Gingras A, Myers C, Andrews B, Boone C, Moffat J
Nature Metabolism 2020;2(6):499-513
Nature Metabolism 2020;2(6):499-513
Wdpcp, a PCP Protein Required for Ciliogenesis, Regulates Directional Cell Migration and Cell Polarity by Direct Modulation of the Actin Cytoskeleton
Scott M, Cui C, Chatterjee B, Lozito T, Zhang Z, Francis R, Yagi H, Swanhart L, Sanker S, Francis D, Yu Q, San Agustin J, Puligilla C, Chatterjee T, Tansey T, Liu X, Kelley M, Spiliotis E, Kwiatkowski A, Tuan R, Pazour G, Hukriede N, Lo C
PLoS Biology 2013;11(11):e1001720
PLoS Biology 2013;11(11):e1001720
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Enhanced validation
- Submitted by
- klas2
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot of cell lysate from U-2 OS cells transfected with either siRNA targeting GOLGA2 or control siRNA. Lane 1: Marker (250, 130, 95, 72, 55, 36, 28, 17, 10) Lane 2: Cell lysate from U-2OS cells transfected with siRNA targeting GOLGA2 Lane 3: N/A Lane 4: Cell lysate from U-2OS cells transfected with control siRNA Right image, lane 1-4: loading control
- Sample type
- U-2 OS
- Primary Ab dilution
- 1:203
- Conjugate
- Horseradish Peroxidase
- Secondary Ab
- Secondary Ab
- Secondary Ab dilution
- 1:3000
- Knockdown/Genetic Approaches Application
- Western blot
