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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunocytochemistry [1]
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- Product number
- PA1-077-A647 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GM130 Polyclonal Antibody, Alexa Fluor™ 647
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- PA1077A647 detects GM130 from human samples. The PA1077A647 immunogen is a synthetic peptide corresponding to residues G(368) Q V M E S V R Q L Q M E R D K(383) of human GM130.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Red dye
- Isotype
- IgG
- Vial size
- 50 µL
- Concentration
- 1 mg/mL
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references A MST1-FOXO1 cascade establishes endothelial tip cell polarity and facilitates sprouting angiogenesis.
Kim YH, Choi J, Yang MJ, Hong SP, Lee CK, Kubota Y, Lim DS, Koh GY
Nature communications 2019 Feb 19;10(1):838
Nature communications 2019 Feb 19;10(1):838
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of GM-130 (red) in HeLa cells. The cells were fixed with 4% Paraformaldehyde in PBS for 15 minutes at room temperature, and blocked with 3% BSA in PBS (Product # 37525) for 30 minutes at room temperature. Cells were stained with a GM-130 Polyclonal Antibody, AlexaFluor 647 conjugate (Product # PA1-077-A647) at a dilution of 2.5 µg/mL in blocking buffer for 1 hour at room temperature protected from light. Nuclei (blue) were stained with Hoechst Dye (Product # 62249) at a dilution of 1:10,000 in blocking buffer. Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification.
- Conjugate
- Red dye
