RQ4570

antibody from NSJ Bioreagents

Targeting: GOLGA2 GM130, golgin-95

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

RQ4570

Provider

NSJ Bioreagents

Product name

GM130 Antibody / GOLGA2

Antibody type

Polyclonal

Description

This highly specific GM130 antibody is suitable for use in Western blot/Immunohistochemistry/Flow cytometry/Direct ELISA applications with human samples.

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Vial size

100 ug

Concentration

0.5mg/ml if reconstituted with 0.2ml sterile DI water

Storage

After reconstitution, the GM130 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human 1) placenta, 2) A549, 3) K562, 4) HL-60, 5) MCF-7 and 6) Caco-2 cell lysate with GM130 antibody at 0.5ug/ml. Predicted molecular weight ~130 kDa.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human 1) placenta, 2) A549, 3) K562, 4) HL-60, 5) MCF-7 and 6) Caco-2 cell lysate with GM130 antibody at 0.5ug/ml. Predicted molecular weight ~130 kDa.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human 1) HEK293, 2) Jurkat, 3) HeLa, 4) U-87 MG and 5) PC-3 cell lysate with GM130 antibody at 0.5ug/ml. Predicted molecular weight ~130 kDa.

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunofluorescent staining of FFPE human U-2 OS cells with GM130 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human esophagus squama cancer with GM130 antibody at 0.5ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human placenta with GM130 antibody at 0.5ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human tonsil with GM130 antibody at 0.5ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE mouse intestine with GM130 antibody at 0.5ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE mouse spleen with GM130 antibody at 0.5ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE mouse brain with GM130 antibody at 0.5ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE rat brain with GM130 antibody at 0.5ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human placenta with GM130 antibody at 0.5ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human tonsil with GM130 antibody at 0.5ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of frozen human placental tissue with GM130 antibody.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of human PC-3 cells with GM130 antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= GM130 antibody.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of human U-2 OS cells with GM130 antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= GM130 antibody.